Prediction of Y haplogroup by polymerase chain reaction-reverse blot hybridization assay

Sehee Oh, Jungho Kim, Sunyoung Park, Seoyong Kim, Kyungmyung Lee, Yang Han Lee, Si Keun Lim, Hyeyoung Lee

Research output: Contribution to journalArticlepeer-review


Background: The analysis of Y-SNPs from crime scene samples is helpful for investigators in narrowing down suspects by predicting biogeographical ancestry. Objective: In this study, a PCR-reverse blot hybridization assay (REBA) for predicting Y-chromosome haplogroups was employed to determine the major haplogroups worldwide, including AB, DE, C, C3, F, K, NO, O, O2, and O3 and evaluated. Methods: The REBA detects nine biallelic Y chromosome markers (M9, M89, M122, M145, M175, M214, M217, P31, and RPS4Y711) simultaneously using multiple probes. Results: The REBA for Y-single nucleotide polymorphisms (SNP) genotyping was performed using 40 DNA samples from Asians—14 Koreans, 10 Indonesians, six Chineses, six Thais, and four Mongolians. 40 Asian samples were identified as haplogroup O2 (40%), O3 (32.5%), C3 (17.5%), O (7.5%) and K (2.5%). These cases were confirmed by DNA sequence analysis (κ = 1.00; P < 0.001). Conclusion: PCR-REBA is a rapid and reliable method that complements other SNP detection methods. Therefore, implementing REBA for Y-SNP testing may be a useful tool in predicting Y-chromosome haplogroups.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalGenes and Genomics
Issue number3
Publication statusPublished - 2019 Mar 1

Bibliographical note

Funding Information:
Acknowledgements This work was supported by the National Forensic Service (2017-DNA-03), Ministry of the Interior and Safety, Republic of Korea.

Publisher Copyright:
© 2018, The Genetics Society of Korea and Springer Nature B.V.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics


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