Portable in situ fluorescence cytometry of microscale cell-based assays

Daniel A. Tatosian; Michael L. Shuler; Donghyun Kim

doi:10.1364/OL.30.001689

Portable in situ fluorescence cytometry of microscale cell-based assays

Daniel A. Tatosian, Michael L. Shuler, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (μCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a μCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the μCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.

Original language	English
Pages (from-to)	1689-1691
Number of pages	3
Journal	Optics Letters
Volume	30
Issue number	13
DOIs	https://doi.org/10.1364/OL.30.001689
Publication status	Published - 2005 Jul 1

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OL.30.001689

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Portable in situ fluorescence cytometry of microscale cell-based assays

Abstract

All Science Journal Classification (ASJC) codes

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