Pooled nucleic acid testing to identify antiretroviral treatment failure during HIV infection in Seoul, South Korea

Sun Bean Kim, Hye Won Kim, Hyon Suk Kim, Hea Won Ann, Jae Kyoung Kim, Heun Choi, Min Hyung Kim, Je Eun Song, Jin Young Ahn, Nam Su Ku, Dong Hyun Oh, Yong Chan Kim, Su Jin Jeong, Sang Hoon Han, June Myung Kim, Davey M. Smith, Jun Yong Choi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Background: There have been various efforts to identify less costly but still accurate methods for monitoring the response to HIV treatment. We evaluated a pooling method to determine if this could improve screening efficiency and reduce costs while maintaining accuracy in Seoul, South Korea. Methods: We conducted the first prospective study of pooled nucleic acid testing (NAT) using a 5 minipool + algorithm strategy versus individual viral load testing for patients receiving antiretroviral therapy (ART) between November 2011 and August 2012 at an urban hospital in Seoul, South Korea. The viral load assay used has a lower level of detection of 20 HIV RNA copies/ml, and the cost per assay is US$ 136. The 5 minipool +algorithm strategy was applied and 43 pooled samples were evaluated. The relative efficiency and accuracy of the pooled NAT were compared with those of individual testing. Results: Using the individual viral load assay, 15 of 215 (7%) plasma samples had more than 200 HIV RNA copies/ml. The pooled NAT using the 5 minipool + algorithm strategy was applied to 43 pooled samples; 111 tests were needed to test all samples when virologic failure was defined at HIV RNA ≥ 200 copies/ml. Therefore, 104 tests were saved over individual testing, with a relative efficiency of 0.48. When evaluating costs, a total of US$ 14,144 was saved for 215 individual samples during 10 months. The negative predictive value was 99.5% for all samples with HIV RNA ≥ 200 copies/ml. Conclusions: The pooled NAT with 5 minipool + algorithm strategy seems to be a very promising approach to effectively monitor patients receiving ART and to save resources.

Original languageEnglish
Pages (from-to)136-140
Number of pages5
JournalScandinavian Journal of Infectious Diseases
Issue number2
Publication statusPublished - 2014 Feb

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2011-220-E00015), a grant from the Chronic Infectious Disease Cohort (4800-4859-304-260) from Korea Centers for Disease Control and Prevention, and the US National Institutes of Health AI100665, AI080353, MH83552, AI36214, and AI47745.

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases


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