Poly(ethylene glycol) hydrogel microstructures encapsulating living cells

Won Gun Koh, Alexander Revzin, Michael V. Pishko

Research output: Contribution to journalArticlepeer-review

230 Citations (Scopus)


We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 μm in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 μm allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.

Original languageEnglish
Pages (from-to)2459-2462
Number of pages4
Issue number7
Publication statusPublished - 2002 Apr 2

All Science Journal Classification (ASJC) codes

  • General Materials Science
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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