Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules

Wonju Lee; Youngjin Oh; Kyujung Kim; Yoshiaki Kinosita; Nagisa Mikami; Takayuki Nishizaka; Donghyun Kim

doi:10.1117/12.2212903

Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules

Wonju Lee, Youngjin Oh, Kyujung Kim, Yoshiaki Kinosita, Nagisa Mikami, Takayuki Nishizaka, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this presentation, we explore the feasibility of plasmonic nanohole-based sub-diffraction-limited nanoscopy for biomolecular imaging. The technique utilizes near-field distribution localized by surface plasmon localization on metallic nanoholes which is used to sample molecular fluorescence. The optimum geometry of nanohole arrays was determined by numerical analysis. The localization sampling was applied to reconstructing sub-diffraction-limited images of gliding microtubules with a 76 nm effective resolution in the lateral direction. Extraordinary light transmission was also employed to address enhancement of axial resolution using nanohole arrays, based on which extraction of gliding motions of bacteria was demonstrated with an axial resolution down to 50 nm.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Editors	Alexander N. Cartwright, Dan V. Nicolau
Publisher	SPIE
ISBN (Electronic)	9781628419559
DOIs	https://doi.org/10.1117/12.2212903
Publication status	Published - 2016
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States Duration: 2016 Feb 15 → 2016 Feb 17

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9721
ISSN (Print)	1605-7422

Other

Other	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
Country/Territory	United States
City	San Francisco
Period	16/2/15 → 16/2/17

Bibliographical note

Publisher Copyright:
© 2016 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.2212903

Cite this

Lee, W., Oh, Y., Kim, K., Kinosita, Y., Mikami, N., Nishizaka, T., & Kim, D. (2016). Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules. In A. N. Cartwright, & D. V. Nicolau (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII Article 972107 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9721). SPIE. https://doi.org/10.1117/12.2212903

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abstract = "In this presentation, we explore the feasibility of plasmonic nanohole-based sub-diffraction-limited nanoscopy for biomolecular imaging. The technique utilizes near-field distribution localized by surface plasmon localization on metallic nanoholes which is used to sample molecular fluorescence. The optimum geometry of nanohole arrays was determined by numerical analysis. The localization sampling was applied to reconstructing sub-diffraction-limited images of gliding microtubules with a 76 nm effective resolution in the lateral direction. Extraordinary light transmission was also employed to address enhancement of axial resolution using nanohole arrays, based on which extraction of gliding motions of bacteria was demonstrated with an axial resolution down to 50 nm.",

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Lee, W, Oh, Y, Kim, K, Kinosita, Y, Mikami, N, Nishizaka, T & Kim, D 2016, Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules. in AN Cartwright & DV Nicolau (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII., 972107, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9721, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, San Francisco, United States, 16/2/15. https://doi.org/10.1117/12.2212903

Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules. / Lee, Wonju; Oh, Youngjin; Kim, Kyujung et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. ed. / Alexander N. Cartwright; Dan V. Nicolau. SPIE, 2016. 972107 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9721).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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Lee W, Oh Y, Kim K, Kinosita Y, Mikami N, Nishizaka T et al. Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules. In Cartwright AN, Nicolau DV, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII. SPIE. 2016. 972107. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2212903

Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules

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