Plasmonic Annular Nanotrenches with 1 nm Nanogaps for Detection of SARS-CoV-2 Using SERS-Based Immunoassay

Sungwoo Lee; Seonghyeon Lee; Woongkyu Park; Soohyun Lee; Sunwoo Kwon; Myeong Jin Oh; Mohammad Navid Haddadnezhad; Insub Jung; Bohyeon Kim; Jieun Park; Kyu Sung Shin; Hyungdon Lee; Junsang Yoo; Won Keun Kim; Sungho Park

doi:10.1021/acs.nanolett.4c00451

Plasmonic Annular Nanotrenches with 1 nm Nanogaps for Detection of SARS-CoV-2 Using SERS-Based Immunoassay

Sungwoo Lee, Seonghyeon Lee, Woongkyu Park, Soohyun Lee, Sunwoo Kwon, Myeong Jin Oh, Mohammad Navid Haddadnezhad, Insub Jung, Bohyeon Kim, Jieun Park, Kyu Sung Shin, Hyungdon Lee, Junsang Yoo, Won Keun Kim, Sungho Park

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

This study represents the synthesis of a novel class of nanoparticles denoted as annular Au nanotrenches (AANTs). AANTs are engineered to possess embedded, narrow circular nanogaps with dimensions of approximately 1 nm, facilitating near-field focusing for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via a surface-enhanced Raman scattering (SERS)-based immunoassay. Notably, AANTs exhibited an exceedingly low limit of detection (LOD) of 1 fg/mL for SARS-CoV-2 spike glycoproteins, surpassing the commercially available enzyme-linked immunosorbent assay (ELISA) by 6 orders of magnitude (1 ng/mL from ELISA). To assess the real-world applicability, a study was conducted on 50 clinical samples using an SERS-based immunoassay with AANTs. The results revealed a sensitivity of 96% and a selectivity of 100%, demonstrating the significantly enhanced sensing capabilities of the proposed approach in comparison to ELISA and commercial lateral flow assay kits.

Original language	English
Pages (from-to)	4233-4240
Number of pages	8
Journal	Nano letters
Volume	24
Issue number	14
DOIs	https://doi.org/10.1021/acs.nanolett.4c00451
Publication status	Published - 2024 Apr 10

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society.

All Science Journal Classification (ASJC) codes

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.4c00451

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title = "Plasmonic Annular Nanotrenches with 1 nm Nanogaps for Detection of SARS-CoV-2 Using SERS-Based Immunoassay",

abstract = "This study represents the synthesis of a novel class of nanoparticles denoted as annular Au nanotrenches (AANTs). AANTs are engineered to possess embedded, narrow circular nanogaps with dimensions of approximately 1 nm, facilitating near-field focusing for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via a surface-enhanced Raman scattering (SERS)-based immunoassay. Notably, AANTs exhibited an exceedingly low limit of detection (LOD) of 1 fg/mL for SARS-CoV-2 spike glycoproteins, surpassing the commercially available enzyme-linked immunosorbent assay (ELISA) by 6 orders of magnitude (1 ng/mL from ELISA). To assess the real-world applicability, a study was conducted on 50 clinical samples using an SERS-based immunoassay with AANTs. The results revealed a sensitivity of 96% and a selectivity of 100%, demonstrating the significantly enhanced sensing capabilities of the proposed approach in comparison to ELISA and commercial lateral flow assay kits.",

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AU - Lee, Soohyun

AU - Kwon, Sunwoo

AU - Oh, Myeong Jin

AU - Haddadnezhad, Mohammad Navid

AU - Jung, Insub

AU - Kim, Bohyeon

AU - Park, Jieun

AU - Shin, Kyu Sung

AU - Lee, Hyungdon

AU - Yoo, Junsang

AU - Kim, Won Keun

AU - Park, Sungho

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AB - This study represents the synthesis of a novel class of nanoparticles denoted as annular Au nanotrenches (AANTs). AANTs are engineered to possess embedded, narrow circular nanogaps with dimensions of approximately 1 nm, facilitating near-field focusing for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via a surface-enhanced Raman scattering (SERS)-based immunoassay. Notably, AANTs exhibited an exceedingly low limit of detection (LOD) of 1 fg/mL for SARS-CoV-2 spike glycoproteins, surpassing the commercially available enzyme-linked immunosorbent assay (ELISA) by 6 orders of magnitude (1 ng/mL from ELISA). To assess the real-world applicability, a study was conducted on 50 clinical samples using an SERS-based immunoassay with AANTs. The results revealed a sensitivity of 96% and a selectivity of 100%, demonstrating the significantly enhanced sensing capabilities of the proposed approach in comparison to ELISA and commercial lateral flow assay kits.

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Plasmonic Annular Nanotrenches with 1 nm Nanogaps for Detection of SARS-CoV-2 Using SERS-Based Immunoassay

Abstract

Bibliographical note

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