Phasor plot analysis using low pass filter for high-speed FLIM

Dongeun Kim; Wonsang Hwang; Youngjae Won; Sucbei Moon; Sang Yoon Lee; Mingu Kang; Won Sub Han; Dug Young Kim

doi:10.1117/12.2509325

Phasor plot analysis using low pass filter for high-speed FLIM

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Mingu Kang, Won Sub Han, Dug Young Kim

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.

Original language	English
Title of host publication	Single Molecule Spectroscopy and Superresolution Imaging XII
Editors	Ingo Gregor, Felix Koberling, Zygmunt K. Gryczynski
Publisher	SPIE
ISBN (Electronic)	9781510624108
DOIs	https://doi.org/10.1117/12.2509325
Publication status	Published - 2019
Event	Single Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States Duration: 2019 Feb 2 → 2019 Feb 3

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10884
ISSN (Print)	1605-7422

Conference

Conference	Single Molecule Spectroscopy and Superresolution Imaging XII 2019
Country/Territory	United States
City	San Francisco
Period	19/2/2 → 19/2/3

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2509325

Cite this

Kim, D., Hwang, W., Won, Y., Moon, S., Lee, S. Y., Kang, M., Han, W. S., & Kim, D. Y. (2019). Phasor plot analysis using low pass filter for high-speed FLIM. In I. Gregor, F. Koberling, & Z. K. Gryczynski (Eds.), Single Molecule Spectroscopy and Superresolution Imaging XII Article 108840P (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884). SPIE. https://doi.org/10.1117/12.2509325

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title = "Phasor plot analysis using low pass filter for high-speed FLIM",

abstract = "Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.",

author = "Dongeun Kim and Wonsang Hwang and Youngjae Won and Sucbei Moon and Lee, {Sang Yoon} and Mingu Kang and Han, {Won Sub} and Kim, {Dug Young}",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Single Molecule Spectroscopy and Superresolution Imaging XII 2019 ; Conference date: 02-02-2019 Through 03-02-2019",

year = "2019",

doi = "10.1117/12.2509325",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Ingo Gregor and Felix Koberling and Gryczynski, {Zygmunt K.}",

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Kim, D, Hwang, W, Won, Y, Moon, S, Lee, SY, Kang, M, Han, WS & Kim, DY 2019, Phasor plot analysis using low pass filter for high-speed FLIM. in I Gregor, F Koberling & ZK Gryczynski (eds), Single Molecule Spectroscopy and Superresolution Imaging XII., 108840P, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10884, SPIE, Single Molecule Spectroscopy and Superresolution Imaging XII 2019, San Francisco, United States, 19/2/2. https://doi.org/10.1117/12.2509325

Phasor plot analysis using low pass filter for high-speed FLIM. / Kim, Dongeun; Hwang, Wonsang; Won, Youngjae et al.
Single Molecule Spectroscopy and Superresolution Imaging XII. ed. / Ingo Gregor; Felix Koberling; Zygmunt K. Gryczynski. SPIE, 2019. 108840P (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Phasor plot analysis using low pass filter for high-speed FLIM

AU - Kim, Dongeun

AU - Hwang, Wonsang

AU - Won, Youngjae

AU - Moon, Sucbei

AU - Lee, Sang Yoon

AU - Kang, Mingu

AU - Han, Won Sub

AU - Kim, Dug Young

PY - 2019

Y1 - 2019

N2 - Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.

AB - Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.

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M3 - Conference contribution

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BT - Single Molecule Spectroscopy and Superresolution Imaging XII

A2 - Gregor, Ingo

A2 - Koberling, Felix

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T2 - Single Molecule Spectroscopy and Superresolution Imaging XII 2019

Y2 - 2 February 2019 through 3 February 2019

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Phasor plot analysis using low pass filter for high-speed FLIM

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