Performance evaluation of the newly developed BD Phoenix NMIC-500 panel using clinical isolates of gram-negative bacilli

Byeol Yi Park, Demiana Mourad, Jun Sung Hong, Eun Jeong Yoon, Dokyun Kim, Hyukmin Lee, Seok Hoon Jeong

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Background: The emergence of carbapenem resistance among gram-negative bacilli (GNB), mediated by carbapenemase production, has necessitated the development of a simple and accurate device for detecting minimum inhibitory concentrations (MICs) and resistance mechanisms, especially carbapenemase production. We evaluated the performance of the BD Phoenix NMIC-500 panel (BD Diagnostic Systems, Sparks, MD, USA) for antimicrobial susceptibility testing (AST) and carbapenemase-producing organism (CPO) detection. Methods: We used 450 non-duplicate clinical GNB isolates from six general hospitals in Korea (409 Enterobacteriaceae and 41 glucose non-fermenting bacilli [GNFB] isolates). AST for meropenem, imipenem, ertapenem, ceftazidime, and ceftazidime/avibactam, and CPO detection were performed using the Phoenix NMIC-500 panel. Broth microdilution was used as the reference method for AST. The rates of categorical agreement (CA), essential agreement (EA), minor error (mE), major error (ME), and very major error (VME) were calculated in each antimicrobial. In addition, PCR and sequencing were performed to evaluate the accuracy of CPO detection by the BD Phoenix NMIC-500 panel, and the rate of correct identification was calculated. Results: The CA rates were >90% for all antimicrobials tested with the Enterobacteriaceae isolates, except for imipenem (87.2%). The GNFB CA rates ranged from 92.7% to 100% for all antimicrobials. The ME rates were 1.7% for Enterobacteriaceae and 0% for GNFB. The panel identified 97.2% (243/250) of the carbapenemase-producing isolates. Conclusions: The BD Phoenix NMIC-500 panel shows promise for AST and CPO detection.

Original languageEnglish
Pages (from-to)470-477
Number of pages8
JournalAnnals of laboratory medicine
Issue number5
Publication statusPublished - 2019

Bibliographical note

Funding Information:
We thank all the colleges in the Research Institute of Bacterial Resistance.

Publisher Copyright:
© Korean Society for Laboratory Medicine

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical


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