PCR-reverse blot hybridization assay for fast and accurate identification of causative species in superficial fungal infections

S. Y. Park, B. K. Kim, H. Y. Wang, S. H. Kim, H. J. Kim, H. Y. Lee, E. H. Choi

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5 Citations (Scopus)


Background Superficial fungal infections are a very common problem in dermatological clinics. The diagnostic method of fungal culture is time-consuming and has inconsistent sensitivity. Therefore, a practical method for rapid and accurate identification of the species causing superficial fungal infections is needed. Aim To compare PCR-reverse blot hybridization assay (PCR-REBA) with conventional fungal diagnostic methods so as to determine the reliability of PCR-REBA for the diagnosis and species identification in superficial fungal infections. Methods Potassium hydroxide (KOH) preparation, fungal culture, conventional real-time PCR and PCR-REBA were used to assess 83 specimens, and the results from each method were compared. Results Of the 83 specimens, 44 specimens that were positive by fungal culture had 62.7% agreement with PCR-REBA. Compared with real-time PCR, there was 68.7% agreement with fungal culture, but 91.6% agreement with PCR-REBA. When the comparison was made using the 55 specimens that gave positive results in both KOH preparation and fungal culture, there was 85.5% agreement with real-time PCR for fungal culture, but 94.5% agreement with PCR-REBA. Conclusions Compared with KOH preparation or fungal culture, PCR-REBA has higher sensitivity and specificity. Therefore, PCR-REBA could be a useful method in clinical settings because it can identify species quickly and accurately, and can also determine the existence of pathogens.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalClinical and Experimental Dermatology
Issue number4
Publication statusPublished - 2016 Jun 1

Bibliographical note

Publisher Copyright:
© 2016 British Association of Dermatologists.

All Science Journal Classification (ASJC) codes

  • Dermatology


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