TY - GEN
T1 - Patterning of alternating proteins inside a microfluidic channel for enhanced tumor cell isolation
AU - Launiere, C. A.
AU - Gaskill, M. M.
AU - Myung, J. H.
AU - Hong, S.
AU - Eddington, D. T.
PY - 2011
Y1 - 2011
N2 - Here we report on a technique to improve the capture rate of an affinity-based microfluidic CTC isolation device by patterning regions of alternating capture proteins. Of the two capture proteins, anti-epithelial cell adhesion molecule (aEpCAM) provides the specificity for CTC capture, while E-selectin endows robustness that increases CTC capture under shear. This patterning technique may also be useful in other biotechnology applications, as it can be used to spatially control the immobilization of any 2 proteins or protein mixtures inside a sealed microfluidic channel.
AB - Here we report on a technique to improve the capture rate of an affinity-based microfluidic CTC isolation device by patterning regions of alternating capture proteins. Of the two capture proteins, anti-epithelial cell adhesion molecule (aEpCAM) provides the specificity for CTC capture, while E-selectin endows robustness that increases CTC capture under shear. This patterning technique may also be useful in other biotechnology applications, as it can be used to spatially control the immobilization of any 2 proteins or protein mixtures inside a sealed microfluidic channel.
UR - http://www.scopus.com/inward/record.url?scp=84883778928&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883778928&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883778928
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1227
EP - 1229
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -