Optimum phase shift for quantitative phase microscopy in volume measurement

Mohammad Reza Jafarfard, Behnam Tayebi, Seungrag Lee, Yoon Sung Bae, Dug Young Kim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Volume measurement of a phase object is one of the most distinctive capabilities of quantitative phase microscopy (QPM). However, the accuracy of a measured volume is limited by the different noises of a measurement system and the finite bandpass filter used in the phase extraction algorithm. In this paper, we analyze the inherent errors in volume measurement with QPM and propose the optimum condition that can minimize these errors. We find that phase information of a sample in the frequency domain nonlinearly oscillates as a function of the phase shift corresponding to the sample and its medium, and that the phase information of a sample inside the bandpass filter can be maximized by a proper phase shift. Through numerical simulations and actual experiments, we demonstrate that the error in phase volume measurement can be effectively reduced by the enhancement of the phase signal inside the bandpass region using an optimum amount of phase, which can be controlled by changing either the medium index or the wavelength of illumination.

Original languageEnglish
Pages (from-to)2429-2436
Number of pages8
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Issue number11
Publication statusPublished - 2014 Nov 1

Bibliographical note

Publisher Copyright:
© 2014 Optical Society of America.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition


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