Optimal conditions for the storage of German cockroach extract

Kyoung Yong Jeong, Jongsun Lee, Ji Eun Yuk, Kyung Hee Park, Jae Hyun Lee, Jung Dong Kim, Jung Won Park

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

allergen extracts are commonly utilized for diagnosis and immunotherapy; however, the stability of protease-rich extracts is important for a precise diagnosis and treatment efficacy. The present study determines the optimal conditions for the storage of German cockroach allergen extract. Cockroach extracts were reconstituted in four buffers: normal saline (NS), 50% glycerol in NS, 0.3% phenol in NS, or 0.3% phenol and 50% glycerol in NS. The extracts in different buffers were stored either at room temperature (18-26°C, RT) or refrigerated (2-8°C). Subsequently, the protein concentration and allergen content (Bla g 1 and Bla g 2) in the extracts were examined for the course of one year. Extract potency was estimated by inhibition ELISA. At least 90.5% protein, 94.4% Bla g 1, 65.2% Bla g 2, and 91.4% potency remained after one year when 50% glycerol nS was added to the extract with refrigeration. However, less than 13.7% protein, 17.1% Bla g 1, 0% Bla g 2 and 32.5% potency were maintained after one year when 50% glycerol nS was not added to the extract and was maintained at RT. The addition of 0.3% phenol NS did not show significant effects on extract stability. The addition of 50% glycerol nS and refrigerated storage temperature were found to be important factors for increasing the shelf life of protease-rich cockroach extract.

Original languageEnglish
Pages (from-to)953-958
Number of pages6
JournalMolecular Medicine Reports
Volume21
Issue number2
DOIs
Publication statusPublished - 2020

Bibliographical note

Publisher Copyright:
© 2020 Spandidos Publications. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

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