Onepot-Seq: capturing single-cell transcriptomes simultaneously in a continuous medium via transient localization of mRNA

Dongju Shin, Jungwon Choi, Ji Hyun Lee, Duhee Bang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The development of single-cell RNA-seq has broadened the spectrum for biological research by providing a high-resolution analysis of cellular heterogeneity. However, the requirement for sophisticated devices for the compartmentalization of cells has limited its widespread applicability. Here, we develop Onepot-Seq, a device-free method, that harnesses the transient localization of mRNA after lysis to capture single-cell transcriptomes simultaneously in a continuous fluid medium. In mixed-species experiments, we obtained high-quality single-cell profiles. Further, cell type-specific poly(A)-conjugated antibodies allow Onepot-Seq to effectively capture target cells in complex populations. Chemical perturbations to cells can be profiled by Onepot-Seq at single-cell resolution. Onepot-Seq should allow routine transcriptional profiling at single-cell resolution, accelerating clinical and scientific discoveries in many fields of science.

Original languageEnglish
Pages (from-to)12621-12635
Number of pages15
JournalNucleic acids research
Volume50
Issue number22
DOIs
Publication statusPublished - 2022 Dec 9

Bibliographical note

Publisher Copyright:
© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

All Science Journal Classification (ASJC) codes

  • Genetics

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