Off-axis self-interference incoherent digital holographic microscopy

Philjun Jeon; Heejung Lee; Byunghwy So; Wonsang Hwang; Yoonsung Bae; Dugyoung Kim

doi:10.1117/12.2251760

Off-axis self-interference incoherent digital holographic microscopy

Philjun Jeon, Heejung Lee, Byunghwy So, Wonsang Hwang, Yoonsung Bae, Dugyoung Kim

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

3D imaging is demanding technology required in fluorescence microscopy. Even though holography is a powerful technique, it could not be used easily in fluorescence microscopy because of low coherence of fluorescence light. Lately, several incoherent holographic methods such as scanning holography, Fresnel in coherent correlation holography (FINCH), and self-interference incoherent digital holography (SIDH) have been proposed. However, these methods have many problems to be overcome for practical applications. For example, DC term removal, twin image ambiguity, and phase unwrapping problems need to be resolved. Off-axis holography is a straightforward solution which can solve most of these problems. We built an off-axis SIDH system for fluorescence imaging, and investigated various conditions and requirements for practical holographic fluorescence microscopy. Our system is based on a modified Michelson interferometer with a flat mirror at one arm and a curved mirror at the other arm of the interferometer. We made a phantom 3D fluorescence object made of 2 single-mode fibers coupled to a single red LED source to mimic 2 fluorescence point sources distributed by a few tens of micrometers apart. A cooled EM-CCD was used to take holograms of these fiber ends which emit only around 180 nW power.

Original language	English
Title of host publication	Practical Holography XXXI
Subtitle of host publication	Materials and Applications
Editors	Hans I. Bjelkhagen, V. Michael Bove
Publisher	SPIE
ISBN (Electronic)	9781510606951
DOIs	https://doi.org/10.1117/12.2251760
Publication status	Published - 2017
Event	SPIE Conference on Practical Holography XXXI: Materials and Applications - San Francisco, United States Duration: 2017 Jan 30 → 2017 Feb 1

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10127
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Other

Other	SPIE Conference on Practical Holography XXXI: Materials and Applications
Country/Territory	United States
City	San Francisco
Period	17/1/30 → 17/2/1

Bibliographical note

Funding Information:
This work was financially supported by the MEST through the National Research Foundation of Korea (Grant No. 2012R1A4A1029061), the Center for Advanced Meta-Materials (CAMM) funded by the Ministry of Science, ICT and Future Planning as Global Frontier Project (CAMM-2014M3A6B3063712), the Technology Innovation Program (10062417) funded by the Ministry of Trade, industry and Energy (MI), and the Ministry of Education Science and Technology of Korea through the BK21 program.

Publisher Copyright:
© 2017 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2251760

Cite this

@inproceedings{30ab3b8dc586425fa8808f0275e2198e,

title = "Off-axis self-interference incoherent digital holographic microscopy",

abstract = "3D imaging is demanding technology required in fluorescence microscopy. Even though holography is a powerful technique, it could not be used easily in fluorescence microscopy because of low coherence of fluorescence light. Lately, several incoherent holographic methods such as scanning holography, Fresnel in coherent correlation holography (FINCH), and self-interference incoherent digital holography (SIDH) have been proposed. However, these methods have many problems to be overcome for practical applications. For example, DC term removal, twin image ambiguity, and phase unwrapping problems need to be resolved. Off-axis holography is a straightforward solution which can solve most of these problems. We built an off-axis SIDH system for fluorescence imaging, and investigated various conditions and requirements for practical holographic fluorescence microscopy. Our system is based on a modified Michelson interferometer with a flat mirror at one arm and a curved mirror at the other arm of the interferometer. We made a phantom 3D fluorescence object made of 2 single-mode fibers coupled to a single red LED source to mimic 2 fluorescence point sources distributed by a few tens of micrometers apart. A cooled EM-CCD was used to take holograms of these fiber ends which emit only around 180 nW power.",

author = "Philjun Jeon and Heejung Lee and Byunghwy So and Wonsang Hwang and Yoonsung Bae and Dugyoung Kim",

note = "Funding Information: This work was financially supported by the MEST through the National Research Foundation of Korea (Grant No. 2012R1A4A1029061), the Center for Advanced Meta-Materials (CAMM) funded by the Ministry of Science, ICT and Future Planning as Global Frontier Project (CAMM-2014M3A6B3063712), the Technology Innovation Program (10062417) funded by the Ministry of Trade, industry and Energy (MI), and the Ministry of Education Science and Technology of Korea through the BK21 program. Publisher Copyright: {\textcopyright} 2017 SPIE.; SPIE Conference on Practical Holography XXXI: Materials and Applications ; Conference date: 30-01-2017 Through 01-02-2017",

year = "2017",

doi = "10.1117/12.2251760",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Bjelkhagen, {Hans I.} and Bove, {V. Michael}",

booktitle = "Practical Holography XXXI",

address = "United States",

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Jeon, P, Lee, H, So, B, Hwang, W, Bae, Y & Kim, D 2017, Off-axis self-interference incoherent digital holographic microscopy. in HI Bjelkhagen & VM Bove (eds), Practical Holography XXXI: Materials and Applications., 101270O, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10127, SPIE, SPIE Conference on Practical Holography XXXI: Materials and Applications, San Francisco, United States, 17/1/30. https://doi.org/10.1117/12.2251760

Off-axis self-interference incoherent digital holographic microscopy. / Jeon, Philjun; Lee, Heejung; So, Byunghwy et al.
Practical Holography XXXI: Materials and Applications. ed. / Hans I. Bjelkhagen; V. Michael Bove. SPIE, 2017. 101270O (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10127).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Off-axis self-interference incoherent digital holographic microscopy

AU - Jeon, Philjun

AU - Lee, Heejung

AU - So, Byunghwy

AU - Hwang, Wonsang

AU - Bae, Yoonsung

AU - Kim, Dugyoung

N1 - Funding Information: This work was financially supported by the MEST through the National Research Foundation of Korea (Grant No. 2012R1A4A1029061), the Center for Advanced Meta-Materials (CAMM) funded by the Ministry of Science, ICT and Future Planning as Global Frontier Project (CAMM-2014M3A6B3063712), the Technology Innovation Program (10062417) funded by the Ministry of Trade, industry and Energy (MI), and the Ministry of Education Science and Technology of Korea through the BK21 program. Publisher Copyright: © 2017 SPIE.

PY - 2017

Y1 - 2017

N2 - 3D imaging is demanding technology required in fluorescence microscopy. Even though holography is a powerful technique, it could not be used easily in fluorescence microscopy because of low coherence of fluorescence light. Lately, several incoherent holographic methods such as scanning holography, Fresnel in coherent correlation holography (FINCH), and self-interference incoherent digital holography (SIDH) have been proposed. However, these methods have many problems to be overcome for practical applications. For example, DC term removal, twin image ambiguity, and phase unwrapping problems need to be resolved. Off-axis holography is a straightforward solution which can solve most of these problems. We built an off-axis SIDH system for fluorescence imaging, and investigated various conditions and requirements for practical holographic fluorescence microscopy. Our system is based on a modified Michelson interferometer with a flat mirror at one arm and a curved mirror at the other arm of the interferometer. We made a phantom 3D fluorescence object made of 2 single-mode fibers coupled to a single red LED source to mimic 2 fluorescence point sources distributed by a few tens of micrometers apart. A cooled EM-CCD was used to take holograms of these fiber ends which emit only around 180 nW power.

AB - 3D imaging is demanding technology required in fluorescence microscopy. Even though holography is a powerful technique, it could not be used easily in fluorescence microscopy because of low coherence of fluorescence light. Lately, several incoherent holographic methods such as scanning holography, Fresnel in coherent correlation holography (FINCH), and self-interference incoherent digital holography (SIDH) have been proposed. However, these methods have many problems to be overcome for practical applications. For example, DC term removal, twin image ambiguity, and phase unwrapping problems need to be resolved. Off-axis holography is a straightforward solution which can solve most of these problems. We built an off-axis SIDH system for fluorescence imaging, and investigated various conditions and requirements for practical holographic fluorescence microscopy. Our system is based on a modified Michelson interferometer with a flat mirror at one arm and a curved mirror at the other arm of the interferometer. We made a phantom 3D fluorescence object made of 2 single-mode fibers coupled to a single red LED source to mimic 2 fluorescence point sources distributed by a few tens of micrometers apart. A cooled EM-CCD was used to take holograms of these fiber ends which emit only around 180 nW power.

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M3 - Conference contribution

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ER -

Off-axis self-interference incoherent digital holographic microscopy

Abstract

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Bibliographical note

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