Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

Sung Ho Lee; Seung Hyun Hong; Seung Han Park; Boram Kim; Dong Hee Kim; Seung Woo Park

doi:10.3938/jkps.75.485

Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

Sung Ho Lee, Seung Hyun Hong, Seung Han Park, Boram Kim, Dong Hee Kim, Seung Woo Park

Department of Physics

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a high-speed and high-resolution multimodal nonlinear optical microscope, which can acquire in vivo label and label-free 3D images. Using this microscope, we could successfully observe dynamic images of the zebrafish heartbeat with 33-millisecond intervals without staining process, indicating 1.68 beats per second. In addition, we show that the label-free, high-speed, real-time images can be obtained during somatic cell divisions in the zebrafish’s embryo.

Original language	English
Pages (from-to)	485-489
Number of pages	5
Journal	Journal of the Korean Physical Society
Volume	75
Issue number	6
DOIs	https://doi.org/10.3938/jkps.75.485
Publication status	Published - 2019 Sept 1

Bibliographical note

Funding Information:
This research was supported by the BK21 PLUS program and the Pioneer Research Center Program (20100002212) through the National Research Foundation (NRF) funded by the Ministry of Education of Korea.

Publisher Copyright:
© 2019, The Korean Physical Society.

All Science Journal Classification (ASJC) codes

Physics and Astronomy(all)

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10.3938/jkps.75.485

Cite this

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title = "Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope",

abstract = "We have developed a high-speed and high-resolution multimodal nonlinear optical microscope, which can acquire in vivo label and label-free 3D images. Using this microscope, we could successfully observe dynamic images of the zebrafish heartbeat with 33-millisecond intervals without staining process, indicating 1.68 beats per second. In addition, we show that the label-free, high-speed, real-time images can be obtained during somatic cell divisions in the zebrafish{\textquoteright}s embryo.",

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AU - Kim, Boram

AU - Kim, Dong Hee

AU - Park, Seung Woo

N1 - Funding Information: This research was supported by the BK21 PLUS program and the Pioneer Research Center Program (20100002212) through the National Research Foundation (NRF) funded by the Ministry of Education of Korea. Publisher Copyright: © 2019, The Korean Physical Society.

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Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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