O-Linked N-Acetylglucosamine Modification of Mitochondrial Antiviral Signaling Protein Regulates Antiviral Signaling by Modulating Its Activity

Junghwa Seo, Yun Soo Park, Tae Hyun Kweon, Jingu Kang, Seongjin Son, Han Byeol Kim, Yu Ri Seo, Min Jueng Kang, Eugene C. Yi, Yong Ho Lee, Jin Hong Kim, Boyoun Park, Won Ho Yang, Jin Won Cho

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Post-translational modifications, including O-GlcNAcylation, play fundamental roles in modulating cellular events, including transcription, signal transduction, and immune signaling. Several molecular targets of O-GlcNAcylation associated with pathogen-induced innate immune responses have been identified; however, the direct regulatory mechanisms linking O-GlcNAcylation with antiviral RIG-I-like receptor signaling are not fully understood. In this study, we found that cellular levels of O-GlcNAcylation decline in response to infection with Sendai virus. We identified a heavily O-GlcNAcylated serine-rich region between amino acids 249–257 of the mitochondrial antiviral signaling protein (MAVS); modification at this site disrupts MAVS aggregation and prevents MAVS-mediated activation and signaling. O-GlcNAcylation of the serine-rich region of MAVS also suppresses its interaction with TRAF3; this prevents IRF3 activation and production of interferon-β. Taken together, these results suggest that O-GlcNAcylation of MAVS may be a master regulatory event that promotes host defense against RNA viruses.

Original languageEnglish
Article number589259
JournalFrontiers in Immunology
Volume11
DOIs
Publication statusPublished - 2021 Feb 2

Bibliographical note

Publisher Copyright:
© Copyright © 2021 Seo, Park, Kweon, Kang, Son, Kim, Seo, Kang, Yi, Lee, Kim, Park, Yang and Cho.

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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