Nucleic acid functionalized graphene for biosensing

Alessandra Bonanni, Adriano Ambrosi, Martin Pumera

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)


There is immense demand for complex nanoarchitectures based on graphene nanostructures in the fields of biosensing or nanoelectronics. DNA molecules represent the most versatile and programmable recognition element and can provide a unique massive parallel assembly strategy with graphene nanomaterials. Here we demonstrate a facile strategy for covalent linking of single stranded DNA (ssDNA) to graphene using carbodiimide chemistry and apply it to genosensing. Since graphenes can be prepared by different methods and can contain various oxygen containing groups, we thoroughly investigated the utility of four different chemically modified graphenes for functionalization by ssDNA. The materials were characterized in detail and the different DNA functionalized graphene platforms were then employed for the detection of DNA hybridization and DNA polymorphism by using impedimetric methods. We believe that our findings are very important for the development of novel devices that can be used as alternatives to classical techniques for sensitive and fast DNA analysis. In addition, covalent functionalization of graphene with ssDNA is expected to have broad implications, from biosensing to nanoelectronics and directed, DNA programmable, self-assembly.

Original languageEnglish
Pages (from-to)1668-1673
Number of pages6
JournalChemistry - A European Journal
Issue number6
Publication statusPublished - 2012 Feb 6

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Organic Chemistry


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