Novel biphenyl-oxidizing bacteria and dioxygenase genes from a Korean tidal mudflat

Tae Kwon Lee; Jaejin Lee; Woo Jun Sul; Shoko Iwai; Benli Chai; James M. Tiedje; Joonhong Park

doi:10.1128/AEM.00023-11

Novel biphenyl-oxidizing bacteria and dioxygenase genes from a Korean tidal mudflat

Tae Kwon Lee, Jaejin Lee, Woo Jun Sul, Shoko Iwai, Benli Chai, James M. Tiedje, Joonhong Park

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [¹³C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the 13C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.

Original language	English
Pages (from-to)	3888-3891
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	11
DOIs	https://doi.org/10.1128/AEM.00023-11
Publication status	Published - 2011 Jun

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

Access to Document

10.1128/AEM.00023-11

Cite this

@article{8f2aa8023a9640f2a0aded042ed04729,

title = "Novel biphenyl-oxidizing bacteria and dioxygenase genes from a Korean tidal mudflat",

abstract = "Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [13C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the 13C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.",

author = "Lee, {Tae Kwon} and Jaejin Lee and Sul, {Woo Jun} and Shoko Iwai and Benli Chai and Tiedje, {James M.} and Joonhong Park",

year = "2011",

month = jun,

doi = "10.1128/AEM.00023-11",

language = "English",

volume = "77",

pages = "3888--3891",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Novel biphenyl-oxidizing bacteria and dioxygenase genes from a Korean tidal mudflat

AU - Lee, Tae Kwon

AU - Lee, Jaejin

AU - Sul, Woo Jun

AU - Iwai, Shoko

AU - Chai, Benli

AU - Tiedje, James M.

AU - Park, Joonhong

PY - 2011/6

Y1 - 2011/6

N2 - Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [13C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the 13C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.

AB - Gene-targeted FLX titanium pyrosequencing integrated with stable isotope probing (SIP) using [13C]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (ARHD). More than 80% of the detected ARHD genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. The 16S rRNA sequences enriched in the 13C fraction were from the Betaproteobacteria, bacilli (primarily Paenibacillus-like), and unclassified phyla.

UR - http://www.scopus.com/inward/record.url?scp=79958260979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958260979&partnerID=8YFLogxK

U2 - 10.1128/AEM.00023-11

DO - 10.1128/AEM.00023-11

M3 - Article

C2 - 21498748

AN - SCOPUS:79958260979

SN - 0099-2240

VL - 77

SP - 3888

EP - 3891

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 11

ER -

Novel biphenyl-oxidizing bacteria and dioxygenase genes from a Korean tidal mudflat

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this