Non-labeling immunoassay based on zeta-potential analysis

Eun Hang Lee; Yong Hee Lee; Jae Chul Pyun

doi:10.1016/j.proeng.2012.09.322

Non-labeling immunoassay based on zeta-potential analysis

Eun Hang Lee, Yong Hee Lee, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Conference article › peer-review

3 Citations (Scopus)

Abstract

Immunoassay has been widely used for the detection of target analytes in complex mixtures such as blood by using highly specific antigen-antibody interactions. In this work, antibodies against a target analyte was immobilized to magnetic beads and the change of surface charge of magnetic beads were measured after the binding of target proteins by using a zeta potential analyzer. For the feasibility test of the zeta potential analysis, horseradish peroxidase (HRP) was used as a model target analyte. The application to medical diagnosis was demonstrated by detection of C-reactive protein (CRP) which is known to be a biomarker for inflammatory diseases.

Original language	English
Pages (from-to)	1023-1024
Number of pages	2
Journal	Procedia Engineering
Volume	47
DOIs	https://doi.org/10.1016/j.proeng.2012.09.322
Publication status	Published - 2012
Event	26th European Conference on Solid-State Transducers, EUROSENSOR 2012 - Krakow, Poland Duration: 2012 Sept 9 → 2012 Sept 12

All Science Journal Classification (ASJC) codes

Engineering(all)

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10.1016/j.proeng.2012.09.322

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title = "Non-labeling immunoassay based on zeta-potential analysis",

abstract = "Immunoassay has been widely used for the detection of target analytes in complex mixtures such as blood by using highly specific antigen-antibody interactions. In this work, antibodies against a target analyte was immobilized to magnetic beads and the change of surface charge of magnetic beads were measured after the binding of target proteins by using a zeta potential analyzer. For the feasibility test of the zeta potential analysis, horseradish peroxidase (HRP) was used as a model target analyte. The application to medical diagnosis was demonstrated by detection of C-reactive protein (CRP) which is known to be a biomarker for inflammatory diseases.",

author = "Lee, {Eun Hang} and Lee, {Yong Hee} and Pyun, {Jae Chul}",

year = "2012",

doi = "10.1016/j.proeng.2012.09.322",

language = "English",

volume = "47",

pages = "1023--1024",

journal = "Procedia Engineering",

issn = "1877-7058",

publisher = "Elsevier BV",

note = "26th European Conference on Solid-State Transducers, EUROSENSOR 2012 ; Conference date: 09-09-2012 Through 12-09-2012",

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T1 - Non-labeling immunoassay based on zeta-potential analysis

AU - Lee, Eun Hang

AU - Lee, Yong Hee

AU - Pyun, Jae Chul

PY - 2012

Y1 - 2012

N2 - Immunoassay has been widely used for the detection of target analytes in complex mixtures such as blood by using highly specific antigen-antibody interactions. In this work, antibodies against a target analyte was immobilized to magnetic beads and the change of surface charge of magnetic beads were measured after the binding of target proteins by using a zeta potential analyzer. For the feasibility test of the zeta potential analysis, horseradish peroxidase (HRP) was used as a model target analyte. The application to medical diagnosis was demonstrated by detection of C-reactive protein (CRP) which is known to be a biomarker for inflammatory diseases.

AB - Immunoassay has been widely used for the detection of target analytes in complex mixtures such as blood by using highly specific antigen-antibody interactions. In this work, antibodies against a target analyte was immobilized to magnetic beads and the change of surface charge of magnetic beads were measured after the binding of target proteins by using a zeta potential analyzer. For the feasibility test of the zeta potential analysis, horseradish peroxidase (HRP) was used as a model target analyte. The application to medical diagnosis was demonstrated by detection of C-reactive protein (CRP) which is known to be a biomarker for inflammatory diseases.

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DO - 10.1016/j.proeng.2012.09.322

M3 - Conference article

AN - SCOPUS:84891708704

SN - 1877-7058

VL - 47

SP - 1023

EP - 1024

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 26th European Conference on Solid-State Transducers, EUROSENSOR 2012

Y2 - 9 September 2012 through 12 September 2012

ER -

Non-labeling immunoassay based on zeta-potential analysis

Abstract

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