NMR Spectroscopy in Structural Genomics

Weontae Lee; Adelinda Yee; Cheryl H. Arrowsmith

doi:10.1201/9780849361432-7

NMR Spectroscopy in Structural Genomics

Weontae Lee, Adelinda Yee, Cheryl H. Arrowsmith

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

NMR spectroscopy is an alternative method to X-ray crystallography for the structure determination of small to medium sized proteins (<25 kDa) in aqueous or micellar solutions. Recent progress in computational and experimental NMR techniques has improved the efficiency of biological NMR research [1-4]. Ever improving strategies for uniform and selective biosynthetic incorporation of stable isotopes (15N, 13C and 2H) into recombinant proteins combined with newer multidimensional (3-D and 4-D) heteronuclear NMR experiments allows one to resolve resonance overlap for larger proteins and complexes than ever before.

Original language	English
Title of host publication	Structural Genomics and High Throughput Structural Biology
Publisher	CRC Press
Pages	49-60
Number of pages	12
ISBN (Electronic)	9780849361432
ISBN (Print)	9780824753351
DOIs	https://doi.org/10.1201/9780849361432-7
Publication status	Published - 2005 Jan 1

Bibliographical note

Publisher Copyright:
© 2006 by Taylor and Francis Group, LLC.

All Science Journal Classification (ASJC) codes

General Medicine
General Biochemistry,Genetics and Molecular Biology

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10.1201/9780849361432-7

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AU - Yee, Adelinda

AU - Arrowsmith, Cheryl H.

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AB - NMR spectroscopy is an alternative method to X-ray crystallography for the structure determination of small to medium sized proteins (<25 kDa) in aqueous or micellar solutions. Recent progress in computational and experimental NMR techniques has improved the efficiency of biological NMR research [1-4]. Ever improving strategies for uniform and selective biosynthetic incorporation of stable isotopes (15N, 13C and 2H) into recombinant proteins combined with newer multidimensional (3-D and 4-D) heteronuclear NMR experiments allows one to resolve resonance overlap for larger proteins and complexes than ever before.

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BT - Structural Genomics and High Throughput Structural Biology

PB - CRC Press

ER -

NMR Spectroscopy in Structural Genomics

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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