Neonatal intensive care unit outbreak caused by a strain of Klebsiella oxytoca resistant to aztreonam due to overproduction of chromosomal β-lactamase

S. H. Jeong; W. M. Kim; C. L. Chang; J. M. Kim; K. Lee; Y. Chong; H. Y. Hwang; Y. W. Baek; H. K. Chung; I. G. Woo; J. Y. Ku

doi:10.1053/jhin.2001.1018

Neonatal intensive care unit outbreak caused by a strain of Klebsiella oxytoca resistant to aztreonam due to overproduction of chromosomal β-lactamase

S. H. Jeong, W. M. Kim, C. L. Chang, J. M. Kim, K. Lee, Y. Chong, H. Y. Hwang, Y. W. Baek, H. K. Chung, I. G. Woo, J. Y. Ku

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Klebsiella oxytoca strains resistant to both aztreonam and ceftriaxone were isolated from six neonates in a neonatal intensive care unit and water reservoirs of two humidifiers attached to the neonatal incubators. These isolates were assumed to be of the same clone because they were characterized by the same antimicrobial susceptibility and pulsed field gel electrophoresis patterns. It was established that the drug resistance was attributed to overproduction of chromosomally encoded K1 β-lactamase. It was determined that an isolate (K. oxytoca H1) contained a high enzyme concentration (27 μg/100 μg of protein in enzyme extracts), at least 27 times higher than the control K. oxytoca N1. It was also demonstrated that isolates had a point mutation in the - 35 concensus region of the promotor gene of bla_OXY-2 leading to enzyme overproduction. Outbreaks caused by K1 hyperproducers have not previously been described.

Original language	English
Pages (from-to)	281-288
Number of pages	8
Journal	Journal of Hospital Infection
Volume	48
Issue number	4
DOIs	https://doi.org/10.1053/jhin.2001.1018
Publication status	Published - 2001

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

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10.1053/jhin.2001.1018

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Jeong, S. H., Kim, W. M., Chang, C. L., Kim, J. M., Lee, K., Chong, Y., Hwang, H. Y., Baek, Y. W., Chung, H. K., Woo, I. G., & Ku, J. Y. (2001). Neonatal intensive care unit outbreak caused by a strain of Klebsiella oxytoca resistant to aztreonam due to overproduction of chromosomal β-lactamase. Journal of Hospital Infection, 48(4), 281-288. https://doi.org/10.1053/jhin.2001.1018

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abstract = "Klebsiella oxytoca strains resistant to both aztreonam and ceftriaxone were isolated from six neonates in a neonatal intensive care unit and water reservoirs of two humidifiers attached to the neonatal incubators. These isolates were assumed to be of the same clone because they were characterized by the same antimicrobial susceptibility and pulsed field gel electrophoresis patterns. It was established that the drug resistance was attributed to overproduction of chromosomally encoded K1 β-lactamase. It was determined that an isolate (K. oxytoca H1) contained a high enzyme concentration (27 μg/100 μg of protein in enzyme extracts), at least 27 times higher than the control K. oxytoca N1. It was also demonstrated that isolates had a point mutation in the - 35 concensus region of the promotor gene of blaOXY-2 leading to enzyme overproduction. Outbreaks caused by K1 hyperproducers have not previously been described.",

author = "Jeong, {S. H.} and Kim, {W. M.} and Chang, {C. L.} and Kim, {J. M.} and K. Lee and Y. Chong and Hwang, {H. Y.} and Baek, {Y. W.} and Chung, {H. K.} and Woo, {I. G.} and Ku, {J. Y.}",

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doi = "10.1053/jhin.2001.1018",

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AU - Jeong, S. H.

AU - Kim, W. M.

AU - Chang, C. L.

AU - Kim, J. M.

AU - Lee, K.

AU - Chong, Y.

AU - Hwang, H. Y.

AU - Baek, Y. W.

AU - Chung, H. K.

AU - Woo, I. G.

AU - Ku, J. Y.

PY - 2001

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AB - Klebsiella oxytoca strains resistant to both aztreonam and ceftriaxone were isolated from six neonates in a neonatal intensive care unit and water reservoirs of two humidifiers attached to the neonatal incubators. These isolates were assumed to be of the same clone because they were characterized by the same antimicrobial susceptibility and pulsed field gel electrophoresis patterns. It was established that the drug resistance was attributed to overproduction of chromosomally encoded K1 β-lactamase. It was determined that an isolate (K. oxytoca H1) contained a high enzyme concentration (27 μg/100 μg of protein in enzyme extracts), at least 27 times higher than the control K. oxytoca N1. It was also demonstrated that isolates had a point mutation in the - 35 concensus region of the promotor gene of blaOXY-2 leading to enzyme overproduction. Outbreaks caused by K1 hyperproducers have not previously been described.

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Neonatal intensive care unit outbreak caused by a strain of Klebsiella oxytoca resistant to aztreonam due to overproduction of chromosomal β-lactamase

Abstract

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