Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

Mostafa Kamal Masud; Jongbeom Na; Tzu En Lin; Victor Malgras; Anant Preet; Abu Ali Ibn Sina; Kathleen Wood; Mutasim Billah; Jeonghun Kim; Jungmok You; Kenya Kani; Andrew E. Whitten; Carlos Salomon; Nam Trung Nguyen; Muhammad J.A. Shiddiky; Matt Trau; Md Shahriar A. Hossain; Yusuke Yamauchi

doi:10.1016/j.bios.2020.112429

Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

Mostafa Kamal Masud, Jongbeom Na, Tzu En Lin, Victor Malgras, Anant Preet, Abu Ali Ibn Sina, Kathleen Wood, Mutasim Billah, Jeonghun Kim, Jungmok You, Kenya Kani, Andrew E. Whitten, Carlos Salomon, Nam Trung Nguyen, Muhammad J.A. Shiddiky, Matt Trau, Md Shahriar A. Hossain, Yusuke Yamauchi

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)₆]^3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)₆]^4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.

Original language	English
Article number	112429
Journal	Biosensors and Bioelectronics
Volume	168
DOIs	https://doi.org/10.1016/j.bios.2020.112429
Publication status	Published - 2020 Nov 15

Bibliographical note

Funding Information:
This work is supported by the Australian Research Council ( ARC ) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council ( ARC ) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland ) to M. K. M. C. S. is supported by Lions Medical Research Foundation , National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Científico y Tecnológico ( FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance ( AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland.

Funding Information:
This work is supported by the Australian Research Council (ARC) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council (ARC) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland) to M. K. M. C. S. is supported by Lions Medical Research Foundation, National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico (FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance (AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland.

Publisher Copyright:
© 2020 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2020.112429

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Masud, M. K., Na, J., Lin, T. E., Malgras, V., Preet, A., Ibn Sina, A. A., Wood, K., Billah, M., Kim, J., You, J., Kani, K., Whitten, A. E., Salomon, C., Nguyen, N. T., Shiddiky, M. J. A., Trau, M., Hossain, M. S. A., & Yamauchi, Y. (2020). Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level. Biosensors and Bioelectronics, 168, [112429]. https://doi.org/10.1016/j.bios.2020.112429

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title = "Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level",

abstract = "Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)6]3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)6]4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.",

author = "Masud, {Mostafa Kamal} and Jongbeom Na and Lin, {Tzu En} and Victor Malgras and Anant Preet and {Ibn Sina}, {Abu Ali} and Kathleen Wood and Mutasim Billah and Jeonghun Kim and Jungmok You and Kenya Kani and Whitten, {Andrew E.} and Carlos Salomon and Nguyen, {Nam Trung} and Shiddiky, {Muhammad J.A.} and Matt Trau and Hossain, {Md Shahriar A.} and Yusuke Yamauchi",

note = "Funding Information: This work is supported by the Australian Research Council ( ARC ) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council ( ARC ) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland ) to M. K. M. C. S. is supported by Lions Medical Research Foundation , National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Cient{\'i}fico y Tecnol{\'o}gico ( FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance ( AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. Funding Information: This work is supported by the Australian Research Council (ARC) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council (ARC) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland) to M. K. M. C. S. is supported by Lions Medical Research Foundation, National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico (FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance (AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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month = nov,

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doi = "10.1016/j.bios.2020.112429",

language = "English",

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Masud, MK, Na, J, Lin, TE, Malgras, V, Preet, A, Ibn Sina, AA, Wood, K, Billah, M, Kim, J, You, J, Kani, K, Whitten, AE, Salomon, C, Nguyen, NT, Shiddiky, MJA, Trau, M, Hossain, MSA & Yamauchi, Y 2020, 'Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level', Biosensors and Bioelectronics, vol. 168, 112429. https://doi.org/10.1016/j.bios.2020.112429

TY - JOUR

T1 - Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

AU - Masud, Mostafa Kamal

AU - Na, Jongbeom

AU - Lin, Tzu En

AU - Malgras, Victor

AU - Preet, Anant

AU - Ibn Sina, Abu Ali

AU - Wood, Kathleen

AU - Billah, Mutasim

AU - Kim, Jeonghun

AU - You, Jungmok

AU - Kani, Kenya

AU - Whitten, Andrew E.

AU - Salomon, Carlos

AU - Nguyen, Nam Trung

AU - Shiddiky, Muhammad J.A.

AU - Trau, Matt

AU - Hossain, Md Shahriar A.

AU - Yamauchi, Yusuke

N1 - Funding Information: This work is supported by the Australian Research Council ( ARC ) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council ( ARC ) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland ) to M. K. M. C. S. is supported by Lions Medical Research Foundation , National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Científico y Tecnológico ( FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance ( AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. Funding Information: This work is supported by the Australian Research Council (ARC) Discovery Project (DP190102944). This work is partially supported by the Australian Research Council (ARC) Future Fellow (FT150100479) to Y. Y. and Australian Government Research Training Program (RTP) Scholarship (the University of Queensland) to M. K. M. C. S. is supported by Lions Medical Research Foundation, National Health and Medical Research Council - Medical Research Future Fund (1199984), and Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico (FONDECYT 1170809). M. K. M. would also like to thank AINSE Limited for providing financial assistance (AINSE PGRA Award 2018). This work is partially performed at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australian researchers. The authors also acknowledge the facilities, and the scientific and technical assistance, of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/11/15

Y1 - 2020/11/15

N2 - Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)6]3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)6]4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.

AB - Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)6]3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)6]4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.

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Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

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