Nanospeckle illumination microscopy via randomly localized near-field speckle by nanocomposite islands

Hajun Yoo, Hongki Lee, Woo Joong Rhee, Gwiyeong Moon, Changhun Lee, Jeon Soo Shin, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Beyond structured illumination microscopy (SIM) which uses diffraction-limited light illumination, specially designed nanostructures such as metallic nanoantenna arrays generating localized surface plasmon have been developed to expand the frequency information without increasing photon energy. In this study, disordered temperature-annealed nanocomposite islands were used to create random distribution of nanospeckles because nanoisland substrates can be mass-produced in a large observation area by thin film deposition and annealing process. In our nanospeckle illumination microscopy (NanoSIM) system, azimuthal scanning illumination (ASI) on nanoislands creates a randomly localized nearfield distribution that induces an arbitrary number of fluorescence images. By the difficulty of obtaining structured illumination patterns of random nanostructures, images were reconstructed using a modified blind-SIM algorithm which fits well with the ASI system. A 100 nm fluorescent nanobead experiment confirms that NanoSIM provides resolution enhancement of spatial information in good agreement with the results obtained from AFM images. We emphasize that using random nanospeckles of disordered nanocomposite islands can provide highly accessible super-resolution. The results can be applied to imaging and sensing techniques, such as switching-based multi-channel microscopy.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX
EditorsDror Fixler, Ewa M. Goldys, Sebastian Wachsmann-Hogiu
PublisherSPIE
ISBN (Electronic)9781510648234
DOIs
Publication statusPublished - 2022
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX 2022 - Virtual, Online
Duration: 2022 Feb 202022 Feb 24

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11976
ISSN (Print)1605-7422

Conference

ConferenceNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIX 2022
CityVirtual, Online
Period22/2/2022/2/24

Bibliographical note

Publisher Copyright:
Copyright © 2022 SPIE.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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