Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors

We envisioned that label-free control of the transport of cells in two dimensions through receptor-ligand interactions would enable simple separation systems that are easy to implement yet retain the specificity of receptor-ligand interactions. Here we demonstrate nanomechanical control of cell transport in two dimensions via transient receptor-ligand adhesive bonds by patterning of receptors that direct cell rolling through an edge effect HL-60 cells rolling on P-selectin receptor patterns were deflected at angles of 5-10° with respect to their direction of travel. Absence of this effect in the case of rigid microsphere models of cell rolling suggests that this two-dimensional motion depends on nanomechanical properties of the rolling cell. This work suggests the feasibility of simple continuous-flow microfluidic cell separation systems that minimize processing steps and yet retain the specificity of receptor-ligand interactions.