Nanoclay-Polyamine Composite Hydrogel for Topical Delivery of Nitric Oxide Gas via Innate Gelation Characteristics of Laponite

Kyungtae Park; Jonathan I. Dawson; Richard O.C. Oreffo; Yang Hee Kim; Jinkee Hong

doi:10.1021/acs.biomac.0c00086

Nanoclay-Polyamine Composite Hydrogel for Topical Delivery of Nitric Oxide Gas via Innate Gelation Characteristics of Laponite

Kyungtae Park, Jonathan I. Dawson, Richard O.C. Oreffo, Yang Hee Kim, Jinkee Hong

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite-polyamine (LP-PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP-PAn composite. We investigated the conformation of the LP-PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV-vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.

Original language	English
Pages (from-to)	2096-2103
Number of pages	8
Journal	Biomacromolecules
Volume	21
Issue number	6
DOIs	https://doi.org/10.1021/acs.biomac.0c00086
Publication status	Published - 2020 Jun 8

Bibliographical note

Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1).

Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1).

Publisher Copyright:
Copyright © 2020 American Chemical Society.

All Science Journal Classification (ASJC) codes

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

Access to Document

10.1021/acs.biomac.0c00086

Cite this

@article{12aebf4f8a1a47048de4e693976a494e,

title = "Nanoclay-Polyamine Composite Hydrogel for Topical Delivery of Nitric Oxide Gas via Innate Gelation Characteristics of Laponite",

abstract = "Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite-polyamine (LP-PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP-PAn composite. We investigated the conformation of the LP-PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV-vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.",

author = "Kyungtae Park and Dawson, {Jonathan I.} and Oreffo, {Richard O.C.} and Kim, {Yang Hee} and Jinkee Hong",

note = "Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1). Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1). Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = jun,

day = "8",

doi = "10.1021/acs.biomac.0c00086",

language = "English",

volume = "21",

pages = "2096--2103",

journal = "Biomacromolecules",

issn = "1525-7797",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - Nanoclay-Polyamine Composite Hydrogel for Topical Delivery of Nitric Oxide Gas via Innate Gelation Characteristics of Laponite

AU - Park, Kyungtae

AU - Dawson, Jonathan I.

AU - Oreffo, Richard O.C.

AU - Kim, Yang Hee

AU - Hong, Jinkee

N1 - Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1). Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI18C2021) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343). This work was supported by the UK-Korea Partnering Award jointly funded by the UK Medical Research Council (MRC) and the Korea Health Industry Development Institute (KHIDI) (grant number MC_PC_18015). J.I.D. gratefully acknowledges fellowship funding from the EPSRC (grant number EP/L010259/1). Publisher Copyright: Copyright © 2020 American Chemical Society.

PY - 2020/6/8

Y1 - 2020/6/8

N2 - Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite-polyamine (LP-PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP-PAn composite. We investigated the conformation of the LP-PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV-vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.

AB - Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite-polyamine (LP-PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP-PAn composite. We investigated the conformation of the LP-PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV-vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.

UR - http://www.scopus.com/inward/record.url?scp=85086051735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85086051735&partnerID=8YFLogxK

U2 - 10.1021/acs.biomac.0c00086

DO - 10.1021/acs.biomac.0c00086

M3 - Article

C2 - 32267672

AN - SCOPUS:85086051735

SN - 1525-7797

VL - 21

SP - 2096

EP - 2103

JO - Biomacromolecules

JF - Biomacromolecules

IS - 6

ER -

Nanoclay-Polyamine Composite Hydrogel for Topical Delivery of Nitric Oxide Gas via Innate Gelation Characteristics of Laponite

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this