Myricetin exerts anti-obesity effects through upregulation of SIRT3 in adipose tissue

Seun Akindehin; Young Suk Jung; Sang Nam Kim; Yeon Ho Son; Icksoo Lee; Je Kyung Seong; Hyun Woo Jeong; Yun Hee Lee

doi:10.3390/nu10121962

Myricetin exerts anti-obesity effects through upregulation of SIRT3 in adipose tissue

Seun Akindehin, Young Suk Jung, Sang Nam Kim, Yeon Ho Son, Icksoo Lee, Je Kyung Seong, Hyun Woo Jeong, Yun Hee Lee

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Myricetin is a biologically active natural polyphenol with beneficial effects on metabolic health. This study aimed to examine the effects of myricetin on the expression levels of genes involved in lipolysis and mitochondrial respiration in adipocytes and the anti-obesity potential of myricetin. The results indicated that myricetin reduced triglyceride (TG) content and increased mitochondrial content and oxygen consumption rate (OCR) in adipocytes in vitro. To determine anti-obesity effect of myricetin, C57BL6/J mice were fed a high-fat diet (HFD) for eight weeks and then treated with myricetin (10 mg/kg) for 2 weeks. The in vivo treatment of myricetin reduced body weight by 11%. Furthermore, it improved the glucose tolerance, and increased fatty acid consumption of HFD-fed mice. Myricetin treatment increased Sirt3 expression and reduced the acetylation of mitochondrial proteins in adipose tissue. Finally, the knockdown of Sirt3 in adipocytes reduced the myricetin-induced increase in mitochondrial oxygen consumption rate by about 27% compared to controls. Our results indicated that myricetin exerted anti-obesity effects through the upregulation of Sirt3 expression and mitochondrial metabolism in adipose tissue.

Original language	English
Article number	1962
Journal	Nutrients
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/nu10121962
Publication status	Published - 2018 Dec 12

Bibliographical note

Funding Information:
Funding: This research was supported by National Research Foundation of Korea grant (No. NRF-2014R1A6A3 A04056472 (YHL)), by the Bio & Medical Technology Development Program of the NRF (No. NRF-2016M3A9D5 A01953818), and by Research Resettlement Fund for the new faculty of Seoul National University. This work was performed within the program of the AMOREPACIFIC Open Research ‘ORT19-01-16E705001’ supported by a grant from AMOREPACIFIC.

Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Food Science
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/nu10121962

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title = "Myricetin exerts anti-obesity effects through upregulation of SIRT3 in adipose tissue",

abstract = "Myricetin is a biologically active natural polyphenol with beneficial effects on metabolic health. This study aimed to examine the effects of myricetin on the expression levels of genes involved in lipolysis and mitochondrial respiration in adipocytes and the anti-obesity potential of myricetin. The results indicated that myricetin reduced triglyceride (TG) content and increased mitochondrial content and oxygen consumption rate (OCR) in adipocytes in vitro. To determine anti-obesity effect of myricetin, C57BL6/J mice were fed a high-fat diet (HFD) for eight weeks and then treated with myricetin (10 mg/kg) for 2 weeks. The in vivo treatment of myricetin reduced body weight by 11%. Furthermore, it improved the glucose tolerance, and increased fatty acid consumption of HFD-fed mice. Myricetin treatment increased Sirt3 expression and reduced the acetylation of mitochondrial proteins in adipose tissue. Finally, the knockdown of Sirt3 in adipocytes reduced the myricetin-induced increase in mitochondrial oxygen consumption rate by about 27% compared to controls. Our results indicated that myricetin exerted anti-obesity effects through the upregulation of Sirt3 expression and mitochondrial metabolism in adipose tissue.",

author = "Seun Akindehin and Jung, {Young Suk} and Kim, {Sang Nam} and Son, {Yeon Ho} and Icksoo Lee and Seong, {Je Kyung} and Jeong, {Hyun Woo} and Lee, {Yun Hee}",

note = "Funding Information: Funding: This research was supported by National Research Foundation of Korea grant (No. NRF-2014R1A6A3 A04056472 (YHL)), by the Bio & Medical Technology Development Program of the NRF (No. NRF-2016M3A9D5 A01953818), and by Research Resettlement Fund for the new faculty of Seoul National University. This work was performed within the program of the AMOREPACIFIC Open Research {\textquoteleft}ORT19-01-16E705001{\textquoteright} supported by a grant from AMOREPACIFIC. Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Seong, Je Kyung

AU - Jeong, Hyun Woo

AU - Lee, Yun Hee

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N2 - Myricetin is a biologically active natural polyphenol with beneficial effects on metabolic health. This study aimed to examine the effects of myricetin on the expression levels of genes involved in lipolysis and mitochondrial respiration in adipocytes and the anti-obesity potential of myricetin. The results indicated that myricetin reduced triglyceride (TG) content and increased mitochondrial content and oxygen consumption rate (OCR) in adipocytes in vitro. To determine anti-obesity effect of myricetin, C57BL6/J mice were fed a high-fat diet (HFD) for eight weeks and then treated with myricetin (10 mg/kg) for 2 weeks. The in vivo treatment of myricetin reduced body weight by 11%. Furthermore, it improved the glucose tolerance, and increased fatty acid consumption of HFD-fed mice. Myricetin treatment increased Sirt3 expression and reduced the acetylation of mitochondrial proteins in adipose tissue. Finally, the knockdown of Sirt3 in adipocytes reduced the myricetin-induced increase in mitochondrial oxygen consumption rate by about 27% compared to controls. Our results indicated that myricetin exerted anti-obesity effects through the upregulation of Sirt3 expression and mitochondrial metabolism in adipose tissue.

AB - Myricetin is a biologically active natural polyphenol with beneficial effects on metabolic health. This study aimed to examine the effects of myricetin on the expression levels of genes involved in lipolysis and mitochondrial respiration in adipocytes and the anti-obesity potential of myricetin. The results indicated that myricetin reduced triglyceride (TG) content and increased mitochondrial content and oxygen consumption rate (OCR) in adipocytes in vitro. To determine anti-obesity effect of myricetin, C57BL6/J mice were fed a high-fat diet (HFD) for eight weeks and then treated with myricetin (10 mg/kg) for 2 weeks. The in vivo treatment of myricetin reduced body weight by 11%. Furthermore, it improved the glucose tolerance, and increased fatty acid consumption of HFD-fed mice. Myricetin treatment increased Sirt3 expression and reduced the acetylation of mitochondrial proteins in adipose tissue. Finally, the knockdown of Sirt3 in adipocytes reduced the myricetin-induced increase in mitochondrial oxygen consumption rate by about 27% compared to controls. Our results indicated that myricetin exerted anti-obesity effects through the upregulation of Sirt3 expression and mitochondrial metabolism in adipose tissue.

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Myricetin exerts anti-obesity effects through upregulation of SIRT3 in adipose tissue

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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