Multiple target loci assembly sequencing (mTAS)

Hyojun Han; Jung Ki Yoon; Byoung Chul Cho; Hwangbeom Kim; Duhee Bang

doi:10.1016/j.ab.2011.04.012

Multiple target loci assembly sequencing (mTAS)

Hyojun Han, Jung Ki Yoon, Byoung Chul Cho, Hwangbeom Kim, Duhee Bang

Research output: Contribution to journal › Article › peer-review

Abstract

Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.

Original language	English
Pages (from-to)	218-220
Number of pages	3
Journal	Analytical Biochemistry
Volume	415
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2011.04.012
Publication status	Published - 2011 Aug 15

Bibliographical note

Funding Information:
This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A101259-1001-0000100), and BK21 program of the Ministry of Education, Science, and Technology. D.B. is a TJ Park junior faculty fellow supported by the Posco TJ Park Foundation.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2011.04.012

Cite this

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title = "Multiple target loci assembly sequencing (mTAS)",

abstract = "Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.",

author = "Hyojun Han and Yoon, {Jung Ki} and Cho, {Byoung Chul} and Hwangbeom Kim and Duhee Bang",

note = "Funding Information: This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A101259-1001-0000100), and BK21 program of the Ministry of Education, Science, and Technology. D.B. is a TJ Park junior faculty fellow supported by the Posco TJ Park Foundation. ",

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doi = "10.1016/j.ab.2011.04.012",

language = "English",

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journal = "Analytical Biochemistry",

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AU - Cho, Byoung Chul

AU - Kim, Hwangbeom

AU - Bang, Duhee

N1 - Funding Information: This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A101259-1001-0000100), and BK21 program of the Ministry of Education, Science, and Technology. D.B. is a TJ Park junior faculty fellow supported by the Posco TJ Park Foundation.

PY - 2011/8/15

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N2 - Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.

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Multiple target loci assembly sequencing (mTAS)

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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