Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar; Chulmin Joo; Chun Zhan; Mikhail Y. Berezin; Walter J. Akers; Samuel Achilefu

doi:10.1117/1.3420209

Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y. Berezin, Walter J. Akers, Samuel Achilefu

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original language	English
Article number	030505
Journal	Journal of Biomedical Optics
Volume	15
Issue number	3
DOIs	https://doi.org/10.1117/1.3420209
Publication status	Published - 2010 May

Bibliographical note

Funding Information:
This study was supported by a grant from the National Institutes of Health (R21/R33 CA12353701). We acknowledge S. Bhaumik, A. Sood, and J. DePuy for help in sample preparation, and P. Codella for performing linear spectroscopy.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.3420209

Cite this

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AU - Akers, Walter J.

AU - Achilefu, Samuel

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Multiphoton microscopy with near infrared contrast agents

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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