Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y. Berezin, Walter J. Akers, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original languageEnglish
Article number030505
JournalJournal of Biomedical Optics
Issue number3
Publication statusPublished - 2010 May

Bibliographical note

Funding Information:
This study was supported by a grant from the National Institutes of Health (R21/R33 CA12353701). We acknowledge S. Bhaumik, A. Sood, and J. DePuy for help in sample preparation, and P. Codella for performing linear spectroscopy.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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