Multifunctional Collagen and Hyaluronic Acid Multilayer Films on Live Mesenchymal Stem Cells

Daheui Choi, Jaeseong Park, Jiwoong Heo, Tong In Oh, Eunah Lee, Jinkee Hong

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment-deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.

Original languageEnglish
Pages (from-to)12264-12271
Number of pages8
JournalACS Applied Materials and Interfaces
Issue number14
Publication statusPublished - 2017 Apr 12

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • General Materials Science


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