More Efficient Media Design for Enhanced Biofouling Control in a Membrane Bioreactor: Quorum Quenching Bacteria Entrapping Hollow Cylinder

Sang H. Lee; Seonki Lee; Kibaek Lee; Chang H. Nahm; Hyeokpil Kwon; Hyun Suk Oh; Young June Won; Kwang Ho Choo; Chung Hak Lee; Pyung Kyu Park

doi:10.1021/acs.est.6b01221

More Efficient Media Design for Enhanced Biofouling Control in a Membrane Bioreactor: Quorum Quenching Bacteria Entrapping Hollow Cylinder

Sang H. Lee, Seonki Lee, Kibaek Lee, Chang H. Nahm, Hyeokpil Kwon, Hyun Suk Oh, Young June Won, Kwang Ho Choo, Chung Hak Lee, Pyung Kyu Park

Division of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

Recently, membrane bioreactors (MBRs) with quorum quenching (QQ) bacteria entrapping beads have been reported as a new paradigm in biofouling control because, unlike conventional post-biofilm control methods, bacterial QQ can inhibit biofilm formation through its combined effects of physical scouring of the membrane and inhibition of quorum sensing (QS). In this study, using a special reporter strain (Escherichia coli JB525), the interaction between QS signal molecules and quorum quenching bacteria entrapping beads (QQ-beads) was elucidated through visualization of the QS signal molecules within a QQ-bead using a fluorescence microscope. As a result, under the conditions considered in this study, the surface area of QQ-media was likely to be a dominant parameter in enhancing QQ activity over total mass of entrapped QQ bacteria because QQ bacteria located near the core of a QQ-bead were unable to display their QQ activities. On the basis of this information, a more efficient QQ-medium, a QQ hollow cylinder (QQ-HC), was designed and prepared. In batch experiments, QQ-HCs showed greater QQ activity than QQ-beads as a result of their higher surface area and enhanced physical washing effect because of their larger impact area against the membrane surface. Furthermore, it was shown that such advantages of QQ-HCs resulted in more effective mitigation of membrane fouling than from QQ-beads in lab-scale continuous MBRs.

Original language	English
Pages (from-to)	8596-8604
Number of pages	9
Journal	Environmental Science and Technology
Volume	50
Issue number	16
DOIs	https://doi.org/10.1021/acs.est.6b01221
Publication status	Published - 2016 Aug 16

Bibliographical note

Funding Information:
This research was supported by the Convergence Technology Program (2015001640001), which is funded by the Korea Ministry of Environment (MOE). The authors thank Prof. Michael Givskov at the University of Copenhagen for kindly providing E. coli JB525.

Publisher Copyright:
© 2016 American Chemical Society.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Environmental Chemistry

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10.1021/acs.est.6b01221

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@article{98f6913dcb5a449897496cb767e7f23d,

title = "More Efficient Media Design for Enhanced Biofouling Control in a Membrane Bioreactor: Quorum Quenching Bacteria Entrapping Hollow Cylinder",

abstract = "Recently, membrane bioreactors (MBRs) with quorum quenching (QQ) bacteria entrapping beads have been reported as a new paradigm in biofouling control because, unlike conventional post-biofilm control methods, bacterial QQ can inhibit biofilm formation through its combined effects of physical scouring of the membrane and inhibition of quorum sensing (QS). In this study, using a special reporter strain (Escherichia coli JB525), the interaction between QS signal molecules and quorum quenching bacteria entrapping beads (QQ-beads) was elucidated through visualization of the QS signal molecules within a QQ-bead using a fluorescence microscope. As a result, under the conditions considered in this study, the surface area of QQ-media was likely to be a dominant parameter in enhancing QQ activity over total mass of entrapped QQ bacteria because QQ bacteria located near the core of a QQ-bead were unable to display their QQ activities. On the basis of this information, a more efficient QQ-medium, a QQ hollow cylinder (QQ-HC), was designed and prepared. In batch experiments, QQ-HCs showed greater QQ activity than QQ-beads as a result of their higher surface area and enhanced physical washing effect because of their larger impact area against the membrane surface. Furthermore, it was shown that such advantages of QQ-HCs resulted in more effective mitigation of membrane fouling than from QQ-beads in lab-scale continuous MBRs.",

author = "Lee, {Sang H.} and Seonki Lee and Kibaek Lee and Nahm, {Chang H.} and Hyeokpil Kwon and Oh, {Hyun Suk} and Won, {Young June} and Choo, {Kwang Ho} and Lee, {Chung Hak} and Park, {Pyung Kyu}",

note = "Funding Information: This research was supported by the Convergence Technology Program (2015001640001), which is funded by the Korea Ministry of Environment (MOE). The authors thank Prof. Michael Givskov at the University of Copenhagen for kindly providing E. coli JB525. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = aug,

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doi = "10.1021/acs.est.6b01221",

language = "English",

volume = "50",

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T1 - More Efficient Media Design for Enhanced Biofouling Control in a Membrane Bioreactor

T2 - Quorum Quenching Bacteria Entrapping Hollow Cylinder

AU - Lee, Sang H.

AU - Lee, Seonki

AU - Lee, Kibaek

AU - Nahm, Chang H.

AU - Kwon, Hyeokpil

AU - Oh, Hyun Suk

AU - Won, Young June

AU - Choo, Kwang Ho

AU - Lee, Chung Hak

AU - Park, Pyung Kyu

N1 - Funding Information: This research was supported by the Convergence Technology Program (2015001640001), which is funded by the Korea Ministry of Environment (MOE). The authors thank Prof. Michael Givskov at the University of Copenhagen for kindly providing E. coli JB525. Publisher Copyright: © 2016 American Chemical Society.

PY - 2016/8/16

Y1 - 2016/8/16

N2 - Recently, membrane bioreactors (MBRs) with quorum quenching (QQ) bacteria entrapping beads have been reported as a new paradigm in biofouling control because, unlike conventional post-biofilm control methods, bacterial QQ can inhibit biofilm formation through its combined effects of physical scouring of the membrane and inhibition of quorum sensing (QS). In this study, using a special reporter strain (Escherichia coli JB525), the interaction between QS signal molecules and quorum quenching bacteria entrapping beads (QQ-beads) was elucidated through visualization of the QS signal molecules within a QQ-bead using a fluorescence microscope. As a result, under the conditions considered in this study, the surface area of QQ-media was likely to be a dominant parameter in enhancing QQ activity over total mass of entrapped QQ bacteria because QQ bacteria located near the core of a QQ-bead were unable to display their QQ activities. On the basis of this information, a more efficient QQ-medium, a QQ hollow cylinder (QQ-HC), was designed and prepared. In batch experiments, QQ-HCs showed greater QQ activity than QQ-beads as a result of their higher surface area and enhanced physical washing effect because of their larger impact area against the membrane surface. Furthermore, it was shown that such advantages of QQ-HCs resulted in more effective mitigation of membrane fouling than from QQ-beads in lab-scale continuous MBRs.

AB - Recently, membrane bioreactors (MBRs) with quorum quenching (QQ) bacteria entrapping beads have been reported as a new paradigm in biofouling control because, unlike conventional post-biofilm control methods, bacterial QQ can inhibit biofilm formation through its combined effects of physical scouring of the membrane and inhibition of quorum sensing (QS). In this study, using a special reporter strain (Escherichia coli JB525), the interaction between QS signal molecules and quorum quenching bacteria entrapping beads (QQ-beads) was elucidated through visualization of the QS signal molecules within a QQ-bead using a fluorescence microscope. As a result, under the conditions considered in this study, the surface area of QQ-media was likely to be a dominant parameter in enhancing QQ activity over total mass of entrapped QQ bacteria because QQ bacteria located near the core of a QQ-bead were unable to display their QQ activities. On the basis of this information, a more efficient QQ-medium, a QQ hollow cylinder (QQ-HC), was designed and prepared. In batch experiments, QQ-HCs showed greater QQ activity than QQ-beads as a result of their higher surface area and enhanced physical washing effect because of their larger impact area against the membrane surface. Furthermore, it was shown that such advantages of QQ-HCs resulted in more effective mitigation of membrane fouling than from QQ-beads in lab-scale continuous MBRs.

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More Efficient Media Design for Enhanced Biofouling Control in a Membrane Bioreactor: Quorum Quenching Bacteria Entrapping Hollow Cylinder

Abstract

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