TY - GEN
T1 - Molecular sensing for biomarkers of invasive cancer cells using localized surface plasmon resonance
AU - Hong, Yoochan
AU - Ku, Minhee
AU - Suh, Jin Suck
AU - Huh, Yong Min
AU - Yoon, Dae Sung
AU - Yang, Jaemoon
PY - 2013
Y1 - 2013
N2 - In this study, we describe the development of cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance (LSPR) that enabling recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of sensing chip was confirmed using various solvents that having different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto surface of GNRs and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.
AB - In this study, we describe the development of cancer biomarker-sensitive nanobiosensor based on localized surface plasmon resonance (LSPR) that enabling recognition for proteolytic activity of membrane type 1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells. First of all, we prepared biomarker-detectable substrate based on gold nanorods (GNRs) using nanoparticle adsorption method. The sensitivity of sensing chip was confirmed using various solvents that having different refractive indexes. Subsequently, MT1-MMP-specific cleavable peptide was conjugated onto surface of GNRs and molecular sensing about proteolytic activity was conducted using MT1-MMP and cell lysates. Collectively, we developed biomarker detectable sensor, which allows for the effective detection of proteolytic activity about MT1-MMP extracted from invasive cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=84883759782&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883759782&partnerID=8YFLogxK
U2 - 10.1117/12.2018326
DO - 10.1117/12.2018326
M3 - Conference contribution
AN - SCOPUS:84883759782
SN - 9780819493040
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Nano-Bio Sensing, Imaging, and Spectroscopy
T2 - SPIE Nano-Bio Sensing, Imaging, and Spectroscopy
Y2 - 20 February 2013 through 23 February 2013
ER -
