Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization

Kiheung Kim; Wonju Lee; Kyungwha Chung; Hongki Lee; Taehwang Son; Youngjin Oh; Yun Feng Xiao; Dong Ha Kim; Donghyun Kim

doi:10.1016/j.bios.2017.04.046

Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization

Kiheung Kim, Wonju Lee, Kyungwha Chung, Hongki Lee, Taehwang Son, Youngjin Oh, Yun Feng Xiao, Dong Ha Kim, Donghyun Kim

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm², an enhancement by more than three orders of magnitude over conventional SPR detection.

Original language	English
Pages (from-to)	89-98
Number of pages	10
Journal	Biosensors and Bioelectronics
Volume	96
DOIs	https://doi.org/10.1016/j.bios.2017.04.046
Publication status	Published - 2017 Oct 15

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) Grants (NRF-2012R1A4A1029061, NRF-2015R1A2A1A10052826,and NRF-2016K2A9A2A06004899). K. Chung and D.H. Kim acknowledge the financial support by the NRF of Korea Grant funded by the Korean Government (2014R1A2A1A09005656).

Publisher Copyright:
© 2017 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2017.04.046

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title = "Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization",

abstract = "In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, an enhancement by more than three orders of magnitude over conventional SPR detection.",

author = "Kiheung Kim and Wonju Lee and Kyungwha Chung and Hongki Lee and Taehwang Son and Youngjin Oh and Xiao, {Yun Feng} and {Ha Kim}, Dong and Donghyun Kim",

note = "Funding Information: This work was supported by the National Research Foundation (NRF) Grants (NRF-2012R1A4A1029061, NRF-2015R1A2A1A10052826,and NRF-2016K2A9A2A06004899). K. Chung and D.H. Kim acknowledge the financial support by the NRF of Korea Grant funded by the Korean Government (2014R1A2A1A09005656). Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

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doi = "10.1016/j.bios.2017.04.046",

language = "English",

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AU - Kim, Kiheung

AU - Lee, Wonju

AU - Chung, Kyungwha

AU - Lee, Hongki

AU - Son, Taehwang

AU - Oh, Youngjin

AU - Xiao, Yun Feng

AU - Ha Kim, Dong

AU - Kim, Donghyun

N1 - Funding Information: This work was supported by the National Research Foundation (NRF) Grants (NRF-2012R1A4A1029061, NRF-2015R1A2A1A10052826,and NRF-2016K2A9A2A06004899). K. Chung and D.H. Kim acknowledge the financial support by the NRF of Korea Grant funded by the Korean Government (2014R1A2A1A09005656). Publisher Copyright: © 2017 Elsevier B.V.

PY - 2017/10/15

Y1 - 2017/10/15

N2 - In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, an enhancement by more than three orders of magnitude over conventional SPR detection.

AB - In this work, we investigate the detection sensitivity of surface plasmon resonance (SPR) biosensors by engineering spatial distribution of electromagnetic near-fields for colocalization with molecular distribution. The light-matter colocalization was based on plasmonic nanolithography, the concept of which was confirmed by detecting streptavidin biotin interactions on triangular nanoaperture arrays after the structure of the aperture arrays was optimized for colocalization efficiency. The colocalization was shown to amplify optical signature significantly and thereby to achieve detection on the order of 100 streptavidin molecules with a binding capacity below 1 fg/mm2, an enhancement by more than three orders of magnitude over conventional SPR detection.

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Molecular overlap with optical near-fields based on plasmonic nanolithography for ultrasensitive label-free detection by light-matter colocalization

Abstract

Bibliographical note

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