Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935

Jang Bin Woo; Hae Na Kang; Eui Jeon Woo; Soo Bok Lee

doi:10.1016/j.foodchem.2013.09.137

Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935

Jang Bin Woo, Hae Na Kang, Eui Jeon Woo, Soo Bok Lee

Department of Food and Nutrition

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichia coli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.

Original language	English
Pages (from-to)	184-187
Number of pages	4
Journal	Food Chemistry
Volume	148
DOIs	https://doi.org/10.1016/j.foodchem.2013.09.137
Publication status	Published - 2014

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology ( 2012R1A1A2005524 ).

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2013.09.137

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title = "Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935",

abstract = "A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichia coli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.",

author = "Woo, {Jang Bin} and Kang, {Hae Na} and Woo, {Eui Jeon} and Lee, {Soo Bok}",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology ( 2012R1A1A2005524 ). ",

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AU - Woo, Jang Bin

AU - Kang, Hae Na

AU - Woo, Eui Jeon

AU - Lee, Soo Bok

N1 - Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology ( 2012R1A1A2005524 ).

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Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935

Abstract

Bibliographical note

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