Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima

Chang Bin Woo; Hae Na Kang; Soo Bok Lee

doi:10.1007/s10068-014-0170-9

Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima

Chang Bin Woo, Hae Na Kang, Soo Bok Lee

Department of Food and Nutrition

Research output: Contribution to journal › Editorial › peer-review

8 Citations (Scopus)

Abstract

A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.

Original language	English
Pages (from-to)	1243-1246
Number of pages	4
Journal	Food Science and Biotechnology
Volume	23
Issue number	4
DOIs	https://doi.org/10.1007/s10068-014-0170-9
Publication status	Published - 2014 Aug

Bibliographical note

Funding Information:
Acknowledgments This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology (2012R1A1A2005524).

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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title = "Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima",

abstract = "A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.",

author = "Woo, {Chang Bin} and Kang, {Hae Na} and Lee, {Soo Bok}",

note = "Funding Information: Acknowledgments This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology (2012R1A1A2005524).",

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AU - Woo, Chang Bin

AU - Kang, Hae Na

AU - Lee, Soo Bok

N1 - Funding Information: Acknowledgments This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Education, Science and Technology (2012R1A1A2005524).

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Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima

Abstract

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