Molecular biology interventions for activity improvement and production of industrial enzymes

Shashi Kant Bhatia, Narisetty Vivek, Vinod Kumar, Neha Chandel, Meenu Thakur, Dinesh Kumar, Yung Hun Yang, Arivalagan Pugazendhi, Gopalakrishnan Kumar

Research output: Contribution to journalReview articlepeer-review

17 Citations (Scopus)


Metagenomics and directed evolution technology have brought a revolution in search of novel enzymes from extreme environment and improvement of existing enzymes and tuning them towards certain desired properties. Using advanced tools of molecular biology i.e. next generation sequencing, site directed mutagenesis, fusion protein, surface display, etc. now researchers can engineer enzymes for improved activity, stability, and substrate specificity to meet the industrial demand. Although many enzymatic processes have been developed up to industrial scale, still there is a need to overcome limitations of maintaining activity during the catalytic process. In this article recent developments in enzymes industrial applications and advancements in metabolic engineering approaches to improve enzymes efficacy and production are reviewed.

Original languageEnglish
Article number124596
JournalBioresource technology
Publication statusPublished - 2021 Mar

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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