Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples

Elisabetta Canetta, Michael Mazilu, Anna Chiara De Luca, Antonia E. Carruthers, Kishan Dholakia, Neilson Sam, Harry Sargeant, Tina Briscoe, C. Simon Herrington, Andrew C. Riches

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64 Citations (Scopus)


Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows that MRS allows discrimination between Raman spectra of SV-HUC-1 and MGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h. We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples.

Original languageEnglish
Article number037002
JournalJournal of Biomedical Optics
Issue number3
Publication statusPublished - 2011 Mar

Bibliographical note

Funding Information:
Human urothelial (SV-HUC-1) and bladder tumor cell (MGH-U1) lines were kindly supplied by Dr. Catherine Reznikoff and Professor John Masters, respectively. The work was partly funded by the CSO and CR-UK/EPSRC/MRC/DoH (England) Imaging Programme. K. D. is a Royal Society-Wolfson Merit Award Holder. A.C.D.L. is an EPSRC research fellow.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials


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