Mitochondrial Cl--Selective Fluorescent Probe for Biological Applications

Sang Hyun Park; Insu Shin; Young Hyun Kim; Injae Shin

doi:10.1021/acs.analchem.0c02658

Mitochondrial Cl^--Selective Fluorescent Probe for Biological Applications

Sang Hyun Park, Insu Shin, Young Hyun Kim, Injae Shin

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Herein we describe the development of the first mitochondrial Cl-selective fluorescent probe, Mito-MQAE, and its applications in biological systems. Fluorescence of Mito-MQAE is insensitive to pH over the physiological pH range and is quenched by Cl- with a Stern-Volmer quenching constant of 201 M-1 at pH 7.0. The results of cell studies using Mito-MQAE show that substances with the ability to disrupt mitochondrial membranes cause increases in the mitochondrial Cl- concentration.

Original language	English
Pages (from-to)	12116-12119
Number of pages	4
Journal	Analytical Chemistry
Volume	92
Issue number	18
DOIs	https://doi.org/10.1021/acs.analchem.0c02658
Publication status	Published - 2020 Sept 15

Bibliographical note

Funding Information:
This work was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT; Grant No. 2020R1A2C3003462).

Publisher Copyright:
Copyright © 2020 American Chemical Society.

All Science Journal Classification (ASJC) codes

Analytical Chemistry

Access to Document

10.1021/acs.analchem.0c02658

Cite this

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title = "Mitochondrial Cl--Selective Fluorescent Probe for Biological Applications",

abstract = "Herein we describe the development of the first mitochondrial Cl-selective fluorescent probe, Mito-MQAE, and its applications in biological systems. Fluorescence of Mito-MQAE is insensitive to pH over the physiological pH range and is quenched by Cl- with a Stern-Volmer quenching constant of 201 M-1 at pH 7.0. The results of cell studies using Mito-MQAE show that substances with the ability to disrupt mitochondrial membranes cause increases in the mitochondrial Cl- concentration.",

author = "Park, {Sang Hyun} and Insu Shin and Kim, {Young Hyun} and Injae Shin",

note = "Funding Information: This work was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT; Grant No. 2020R1A2C3003462). Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",

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AU - Shin, Insu

AU - Kim, Young Hyun

AU - Shin, Injae

N1 - Funding Information: This work was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT; Grant No. 2020R1A2C3003462). Publisher Copyright: Copyright © 2020 American Chemical Society.

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N2 - Herein we describe the development of the first mitochondrial Cl-selective fluorescent probe, Mito-MQAE, and its applications in biological systems. Fluorescence of Mito-MQAE is insensitive to pH over the physiological pH range and is quenched by Cl- with a Stern-Volmer quenching constant of 201 M-1 at pH 7.0. The results of cell studies using Mito-MQAE show that substances with the ability to disrupt mitochondrial membranes cause increases in the mitochondrial Cl- concentration.

AB - Herein we describe the development of the first mitochondrial Cl-selective fluorescent probe, Mito-MQAE, and its applications in biological systems. Fluorescence of Mito-MQAE is insensitive to pH over the physiological pH range and is quenched by Cl- with a Stern-Volmer quenching constant of 201 M-1 at pH 7.0. The results of cell studies using Mito-MQAE show that substances with the ability to disrupt mitochondrial membranes cause increases in the mitochondrial Cl- concentration.

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