Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

Sunok Oh; Dukjin Kang; Sung Min Ahn; Richard J. Simpson; Bong Hee Lee; Myeong Hee Moon

doi:10.1002/jssc.200600394

Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

Sunok Oh, Dukjin Kang, Sung Min Ahn, Richard J. Simpson, Bong Hee Lee, Myeong Hee Moon

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Asymmetrical flow field-flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.

Original language	English
Pages (from-to)	1082-1087
Number of pages	6
Journal	Journal of Separation Science
Volume	30
Issue number	7
DOIs	https://doi.org/10.1002/jssc.200600394
Publication status	Published - 2007 May

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Filtration and Separation

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10.1002/jssc.200600394

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AU - Lee, Bong Hee

AU - Moon, Myeong Hee

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Miniaturized asymmetrical flow field-flow fractionation: Application to biological vesicles

Abstract

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