Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP

Minhee Ku; Hyun Joon Kim; Su Yee Yau; Nara Yoon; Nam Hee Kim; Jong In Yook; Jin Suck Suh; Dae Eun Kim; Jaemoon Yang

doi:10.1002/smll.201803000

Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP

Minhee Ku, Hyun Joon Kim, Su Yee Yau, Nara Yoon, Nam Hee Kim, Jong In Yook, Jin Suck Suh, Dae Eun Kim, Jaemoon Yang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease-mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1-matrix metalloproteinase (MT1-MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1-MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere-attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1-MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM-based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.

Original language	English
Article number	1803000
Journal	Small
Volume	14
Issue number	41
DOIs	https://doi.org/10.1002/smll.201803000
Publication status	Published - 2018 Oct 11

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) grants (2017R1C1B2010867, 2014R1A1A2059806, and 2015R1A2A1A05001887) funded by the Ministry of Science, ICT and Future Planning (MSIP) and the National Research Foundation of Korea (NRF) grant funded by the Korea government(MSIT) (2010-0018289). This work was also supported by a grant from the National R&D Program for Cancer Control (1220100) and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (HI17C2586), funded by the Ministry of Health and Welfare, Republic of Korea.

Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

All Science Journal Classification (ASJC) codes

Biotechnology
Biomaterials
Chemistry(all)
Materials Science(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/smll.201803000

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title = "Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP",

abstract = "Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease-mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1-matrix metalloproteinase (MT1-MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1-MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere-attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1-MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM-based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.",

author = "Minhee Ku and Kim, {Hyun Joon} and Yau, {Su Yee} and Nara Yoon and Kim, {Nam Hee} and Yook, {Jong In} and Suh, {Jin Suck} and Kim, {Dae Eun} and Jaemoon Yang",

note = "Funding Information: This work was supported by the National Research Foundation (NRF) grants (2017R1C1B2010867, 2014R1A1A2059806, and 2015R1A2A1A05001887) funded by the Ministry of Science, ICT and Future Planning (MSIP) and the National Research Foundation of Korea (NRF) grant funded by the Korea government(MSIT) (2010-0018289). This work was also supported by a grant from the National R&D Program for Cancer Control (1220100) and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (HI17C2586), funded by the Ministry of Health and Welfare, Republic of Korea. Publisher Copyright: {\textcopyright} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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AU - Kim, Nam Hee

AU - Yook, Jong In

AU - Suh, Jin Suck

AU - Kim, Dae Eun

AU - Yang, Jaemoon

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PY - 2018/10/11

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N2 - Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease-mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1-matrix metalloproteinase (MT1-MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1-MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere-attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1-MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM-based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.

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Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP

Abstract

Bibliographical note

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UN SDGs

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