In this paper, we describe a simple method for fabricating micropatterned polymeric fibrous scaffolds that are capable of controlling the spatial positioning of mammalian cells. Micropatterned scaffolds were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on electrospun poly(styrene) (PS) fiber matrices, and produced as free-standing and bidirectionally-porous sheets. Clearly defined hydrogel micropatterns incorporating PS fibers were created using photolithography without any residual hydrogel precursor solution remaining in the PS fiber region. Most of the PS fibers were inserted through the side walls of the hydrogel microstructures, leaving no fiber residue on the top surfaces of hydrogel micropatterns. Since the PEG hydrogel showed non-adhesiveness toward proteins and cells, cells selectively adhered and remained viable within the PS fiber region, thereby creating cellular micropatterns. Finally, possible application of this system to cell-based biosensor was demonstrated using cytotoxicity assay.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation (NRF) grant funded by Ministry of Education, Science and Technology (No. 2009-0084190 and R11-2007-050-03002-0 “Active Polymer Center for Pattern Integration at Yonsei University“) and by a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea. The authors are also grateful for the financial support of this research through grants from Seoul Research and Business Development Program (10816).
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry