Micro/nanofluidic device for single-cell-based assay

Kwang Seok Yun; Euisik Yoon

doi:10.1007/s10544-005-6169-5

Micro/nanofluidic device for single-cell-based assay

Kwang Seok Yun, Euisik Yoon

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

In this paper we have proposed and demonstrated a microfluidic device for a fast and parallel single-cell based assay. The proposed device is designed to passively capture single-cells or beads on multiple cell-positioning sites by a pre-defined fluidic stream and inject specific reagents or drugs onto each isolated single-cell. The device consists of surface-modified silicon channels capped with a grooved polydimethylsiloxane (PDMS) cover layer. A cell capture experiment has been performed using polystyrene beads as well as CHO DG44 living cells, and successful positioning of a single-cell on the cell-positioning sites was achieved. Also, we have demonstrated independent drug injection into a specific target cell.

Original language	English
Pages (from-to)	35-40
Number of pages	6
Journal	Biomedical Microdevices
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s10544-005-6169-5
Publication status	Published - 2005 Mar

Bibliographical note

Funding Information:
This research has been supported by the Intelligent Mi-crosystem Center, which carries out one of the 21st century’s Frontier R&D Projects sponsored by the Korea Ministry of Commerce, Industry and Energy. This work was supported by Brain Korea 21 Project, the School of Information Technology, KAIST in 2004. Also, this work has been partially supported by KOSEF through the MICROS center at KAIST.

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Molecular Biology

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10.1007/s10544-005-6169-5

Cite this

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title = "Micro/nanofluidic device for single-cell-based assay",

abstract = "In this paper we have proposed and demonstrated a microfluidic device for a fast and parallel single-cell based assay. The proposed device is designed to passively capture single-cells or beads on multiple cell-positioning sites by a pre-defined fluidic stream and inject specific reagents or drugs onto each isolated single-cell. The device consists of surface-modified silicon channels capped with a grooved polydimethylsiloxane (PDMS) cover layer. A cell capture experiment has been performed using polystyrene beads as well as CHO DG44 living cells, and successful positioning of a single-cell on the cell-positioning sites was achieved. Also, we have demonstrated independent drug injection into a specific target cell.",

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Micro/nanofluidic device for single-cell-based assay

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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