Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

Ann Na Cho, Yoonhee Jin, Yeonjoo An, Jin Kim, Yi Sun Choi, Jung Seung Lee, Junghoon Kim, Won Young Choi, Dong Jun Koo, Weonjin Yu, Gyeong Eon Chang, Dong Yoon Kim, Sung Hyun Jo, Jihun Kim, Sung Yon Kim, Yun Gon Kim, Ju Young Kim, Nakwon Choi, Eunji Cheong, Young Joon KimHyunsoo Shawn Je, Hoon Chul Kang, Seung Woo Cho

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)


Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with brain extracellular matrix significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.

Original languageEnglish
Article number4730
JournalNature communications
Issue number1
Publication statusPublished - 2021 Dec 1

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • General
  • Physics and Astronomy(all)


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