Abstract
Metal-enhanced fluorescence (MEF) of quantum dots (QDs) and its potential application in microarray-based immunoassays was investigated using silver nanoparticles (AgNPs) prepared by the in situ photoreduction of Ag+ inside a multilayer film consisting of poly(ethyleneimine) (PEI) and hyaluronic acid (HA). UV-Vis spectroscopy, X-ray diffraction, atomic force microscopy, scanning electron microscopy, and transmission electron microscopy confirmed the formation of well-dispersed AgNPs within the multilayer films, the thickness and the amount of which depended on the number of HA layers. Using AgNPs-containing hybrid multilayered films, it was possible to observe the MEF effect of adsorbed QDs, which could be tuned by the thickness of interlayer spacer film prepared of the layer-by-layer assembly of PEI and poly(styrene sulfonate). When the MEF-inducing hybrid film was used as a platform for immunoassay, a significant improvement in the fluorescence signal and sensitivity of the biosensing were observed in the presence of AgNPs in comparison with films that did not contain the nanoparticles.
| Original language | English |
|---|---|
| Pages (from-to) | 1355-1364 |
| Number of pages | 10 |
| Journal | Colloid and Polymer Science |
| Volume | 292 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2014 Jun |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) (2011-0022709, 2010K001430 “Converging Research Center Program,” and R11-2007-050-03002-0 “Active Polymer Center for Pattern Integration at Yonsei University”).
All Science Journal Classification (ASJC) codes
- Physical and Theoretical Chemistry
- Polymers and Plastics
- Colloid and Surface Chemistry
- Materials Chemistry