Objective: The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in gingival crevicular fluid (GCF) during root canal treatment (RCT) of nonvital teeth. Study design: Patients scheduled for nonsurgical RCT were prospectively selected; all patients provided informed consent. GCF samples were collected from teeth scheduled for RCT and their contralateral teeth across 3 different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis. Results: Patients' subjective pain levels were significantly related to both MMP-8 and SP levels. MMP-8 and SP levels in GCF were decreased during RCT, and they showed a positive correlation with each other (P <.05). Conclusions: This study demonstrated that periradicular inflammation of endodontic origin can elevate SP and MMP-8 levels in GCF.
|Number of pages||7|
|Journal||Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology|
|Publication status||Published - 2011 Oct|
All Science Journal Classification (ASJC) codes
- Oral Surgery