TY - JOUR
T1 - Mass-producible Nano-featured Polystyrene Surfaces for Regulating the Differentiation of Human Adipose-derived Stem Cells
AU - Park, Kwang Sook
AU - Cha, Kyoung Je
AU - Han, In Bo
AU - Shin, Dong Ah
AU - Cho, Dong Woo
AU - Lee, Soo Hong
AU - Kim, Dong Sung
PY - 2012/11
Y1 - 2012/11
N2 - In this study, we report an efficient and cost-effective method of fabricating polystyrene (PS) nano-featured substrates containing nanopore (NPo) and nanopillar (NPi) arrays based on hot embossing using nickel nano-stamps. We investigate the behavior of adipose-derived stem cells (ASCs), including adhesion, morphology, proliferation and differentiation, on the replicated PS surfaces. Compared to a flat substrate, NPo- and NPi-featured substrates do not alter the morphology of stem cells. However, both NPo- and NPi-featured substrates induce different integrin expression and lower formation of focal adhesion complexes. In addition, ASCs on the NPo-featured substrate exhibit greater adipogenic differentiation, while the NPi-featured substrate induces higher osteogenic differentiation. Artificial nano-featured substrates can be applied for controlling cell behavior. Polystyrene nano-featured substrates with nanopore (NPo) and nanopillar (NPi) arrays are replicated by a polymer molding technique and the behaviors of adipose-derived stem cells (ASCs) are investigated on them. By comparison with a flat substrate, the effect of NPo- and NPi-featured substrates on the attachment, morphology, integrin expression and differentiation of ASCs is shown.
AB - In this study, we report an efficient and cost-effective method of fabricating polystyrene (PS) nano-featured substrates containing nanopore (NPo) and nanopillar (NPi) arrays based on hot embossing using nickel nano-stamps. We investigate the behavior of adipose-derived stem cells (ASCs), including adhesion, morphology, proliferation and differentiation, on the replicated PS surfaces. Compared to a flat substrate, NPo- and NPi-featured substrates do not alter the morphology of stem cells. However, both NPo- and NPi-featured substrates induce different integrin expression and lower formation of focal adhesion complexes. In addition, ASCs on the NPo-featured substrate exhibit greater adipogenic differentiation, while the NPi-featured substrate induces higher osteogenic differentiation. Artificial nano-featured substrates can be applied for controlling cell behavior. Polystyrene nano-featured substrates with nanopore (NPo) and nanopillar (NPi) arrays are replicated by a polymer molding technique and the behaviors of adipose-derived stem cells (ASCs) are investigated on them. By comparison with a flat substrate, the effect of NPo- and NPi-featured substrates on the attachment, morphology, integrin expression and differentiation of ASCs is shown.
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U2 - 10.1002/mabi.201200225
DO - 10.1002/mabi.201200225
M3 - Article
C2 - 23042782
AN - SCOPUS:84868335349
SN - 1616-5187
VL - 12
SP - 1480
EP - 1489
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 11
ER -