Mapping the contact surfaces in the Lamin A: AIMP3 complex by hydrogen/deuterium exchange FT-ICR mass spectrometry

Yeqing Tao, Pengfei Fang, Sunghoon Kim, Min Guo, Nicolas L. Young, Alan G. Marshall

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Aminoacyl-tRNA synthetases-interacting multifunctional protein3 (AIMP3/p18) is involved in the macromolecular tRNA synthetase complex via its interaction with several aminoacyltRNA synthetases. Recent reports reveal a novel function of AIMP3 as a tumor suppressor by accelerating cellular senescence and causing defects in nuclear morphology. AIMP3 specifically mediates degradation of mature Lamin A (LmnA), a major component of the nuclear envelope matrix; however, the mechanism of how AIMP3 interacts with LmnA is unclear. Here we report solution-phase hydrogen/deuterium exchange (HDX) for AIMP3, LmnA, and AIMP3 in association with the LmnA C-terminus. Reversed-phase LC coupled with LTQ 14.5 T Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) results in high mass accuracy and resolving power for comparing the D-uptake profiles for AIMP3, LmnA, and their complex. The results show that the AIMP3-LmnA interaction involves one of the two putative binding sites and an adjacent novel interface on AIMP3. LmnA binds AIMP3 via its extreme C-terminus. Together these findings provide a structural insight for understanding the interaction between AIMP3 and LmnA in AIMP3 degradation.

Original languageEnglish
Article numbere0181869
JournalPloS one
Issue number8
Publication statusPublished - 2017 Aug

Bibliographical note

Publisher Copyright:
© 2017 Tao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General


Dive into the research topics of 'Mapping the contact surfaces in the Lamin A: AIMP3 complex by hydrogen/deuterium exchange FT-ICR mass spectrometry'. Together they form a unique fingerprint.

Cite this