Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples

Young Ki Hahn; Zongwen Jin; Joo H. Kang; Eunkeu Oh; Hak Sung Kim; Jung Tak Jang; Jinwoo Cheon; Hae Sim Park; Je Kyun Park

Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples

Young Ki Hahn, Zongwen Jin, Joo H. Kang, Eunkeu Oh, Hak Sung Kim, Jung Tak Jang, Jinwoo Cheon, Hae Sim Park, Je Kyun Park

Department of Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼10⁴ T m ^-1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL^-1 to 151 pg mL^-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL^-1 to 104 pg mL^-1 (Dermatophagoides farinae).

Original language	English
Pages	320-322
Number of pages	3
Publication status	Published - 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples",

abstract = "This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).",

author = "Hahn, {Young Ki} and Zongwen Jin and Kang, {Joo H.} and Eunkeu Oh and Kim, {Hak Sung} and Jang, {Jung Tak} and Jinwoo Cheon and Park, {Hae Sim} and Park, {Je Kyun}",

year = "2006",

language = "English",

pages = "320--322",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples

AU - Hahn, Young Ki

AU - Jin, Zongwen

AU - Kang, Joo H.

AU - Oh, Eunkeu

AU - Kim, Hak Sung

AU - Jang, Jung Tak

AU - Cheon, Jinwoo

AU - Park, Hae Sim

AU - Park, Je Kyun

PY - 2006

Y1 - 2006

N2 - This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).

AB - This paper reports a novel magnetophoretic immunoassay using a microbead conjugated with superparamagnetic nanoparticles under the enhanced magnetic field gradient. A ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient (∼104 T m -1) and successfully applied to detect allergen-specific IgEs samples obtained from 44 patients' serum in a hospital. The detectable ranges of human IgEs are 10.68 ng mL-1 to 151 pg mL-1 (Dermatophagoides pteronyssinus) and 19.47 ng mL-1 to 104 pg mL-1 (Dermatophagoides farinae).

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UR - http://www.scopus.com/inward/citedby.url?scp=84902491293&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902491293

SP - 320

EP - 322

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Magnetophoretic immunoassay for allergen-specific immunoglobulin E (IgE) in patient samples

Abstract

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All Science Journal Classification (ASJC) codes

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