Abstract
Separation of magnetically labeled cell has been a popular technique in cytometry. Normal Jurkat cells were treated with Chlorohexamide (CHX) and TRAIL to induce apoptosis. Apoptotic cells were treated with biotin functionalized fusion protein, C2A and streptavidin coated magnetic beads. The force balance between magnetic and drag force acting on the cells were calculated. The microfluidic device was designed accordingly. Results from our device were in a good agreement with those of the conventional flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.
| Original language | English |
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| Pages | 416-418 |
| Number of pages | 3 |
| Publication status | Published - 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9 |
Other
| Other | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country/Territory | Japan |
| City | Tokyo |
| Period | 06/11/5 → 06/11/9 |
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering