Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity

Sang Kyou Lee; Bing Su; Stephen E. Maher; Alfred L.M. Bothwell

doi:10.1002/j.1460-2075.1994.tb06493.x

Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity

Sang Kyou Lee, Bing Su, Stephen E. Maher, Alfred L.M. Bothwell

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

To characterize the function of the Ly-6A antigen in T cell activation, antisense Ly-6 RNA was expressed in a stably transfected antigen-specific T cell clone. Reduced Ly-6A expression results in inhibition of responses to antigen, anti-TCR (anti-T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly-6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR β chain. These data demonstrate that Ly-6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.

Original language	English
Pages (from-to)	2167-2176
Number of pages	10
Journal	EMBO Journal
Volume	13
Issue number	9
DOIs	https://doi.org/10.1002/j.1460-2075.1994.tb06493.x
Publication status	Published - 1994

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1002/j.1460-2075.1994.tb06493.x

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abstract = "To characterize the function of the Ly-6A antigen in T cell activation, antisense Ly-6 RNA was expressed in a stably transfected antigen-specific T cell clone. Reduced Ly-6A expression results in inhibition of responses to antigen, anti-TCR (anti-T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly-6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR β chain. These data demonstrate that Ly-6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.",

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AU - Lee, Sang Kyou

AU - Su, Bing

AU - Maher, Stephen E.

AU - Bothwell, Alfred L.M.

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AB - To characterize the function of the Ly-6A antigen in T cell activation, antisense Ly-6 RNA was expressed in a stably transfected antigen-specific T cell clone. Reduced Ly-6A expression results in inhibition of responses to antigen, anti-TCR (anti-T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly-6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR β chain. These data demonstrate that Ly-6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.

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Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity

Abstract

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