Luminol chemiluminescence biosensor for glycated hemoglobin (HbA1c) in human blood samples

Kwang Soo Ahn, Jung Hoon Lee, Jong Myeon Park, Han Nim Choi, Won Yong Lee

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


Luminol chemiluminescence (CL) biosensor based on boronic acid modified gold substrate has been developed for the determination of glycated hemoglobin (HbA1c) in human blood samples. In order to selectively capture HbA1c in sample, carboxy-EG6-undecanethiol was self-assembled on a gold thin-film substrate, followed by covalent coupling of 3-aminophenyl boronic acid (3-APBA). The captured HbA1c containing four iron heme groups plays as a catalyst for luminol CL reaction in the presence of hydrogen peroxide, and thus the luminol CL response is linearly proportional to the amount of HbA1c captured on the biosensor surface. The present biosensor showed linear dynamic range of HbA1c from 2.5% to 17.0%, which well covers the clinically important concentration range. In addition, the present biosensor exhibited negligible response to interfering species such as hemoglobin, fructose, and sorbitol. The present HbA1c biosensor was applied to the determination of HbA1c in human blood samples and the results were well agreed with that obtained with a conventional method.

Original languageEnglish
Article numberBIOSD1501506
Pages (from-to)82-87
Number of pages6
JournalBiosensors and Bioelectronics
Publication statusPublished - 2016 Jan 15

Bibliographical note

Funding Information:
Financial support for this work has been provided by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012-R1A1A2006994 ) and Samsung Electronics.

Publisher Copyright:
© 2015 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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